Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-17
2006-10-17
Woitach, Joseph (Department: 1635)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S455000, C435S456000, C435S325000
Reexamination Certificate
active
07122307
ABSTRACT:
This invention provides for a chamber and system designed for use in assaying drug effects on heart rate. The chamber consists of a series of wells. Cardiac myocytes disaggregated from neonatal animals are plated onto the bottom of each well and grown under standard tissue culture conditions. When drugs are to be assayed, the cells in each well are loaded with a calcium sensitive dye and the beating rate in each is monitored with a photodiode. A drug is added in graded concentrations to each well, and equilibrated and effects on rate are observed. This construct permits use of a cell based bioassay for the study of drugs or agents that may alter cardiac rate. This invention can be used in high throughput screening of drugs to evaluate/predict their effects on cardiac rate and rhythm.
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Cohen Ira S.
Robinson Richard B.
Rosen Michael R.
Yu Han-Gang
Cooper & Dunham LLP
Research Foundation of State University of New York
The Trustees of Columbia University in the City of New York
White, Esq. John P.
Whiteman Brian
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