Methods for purifying and isolating recombinant chondroitinases

Details Methods for purifying and isolating recombinant chondroitinases Methods for purifying and isolating recombinant chondroitinases

2006-10-31

2006-10-31

Saidha, Tekchand (Department: 1652)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

C435S232000, C536S023200

Reexamination Certificate

active

07129335

ABSTRACT:
The invention relates to rationally designed polysaccharide lyases and uses thereof. In particular, the invention relates to modified chondroitinase B. The modified chondroitinase B enzymes of the invention are useful for a variety of purposes, including cleaving and sequencing polysaccharides such as glycosaminoglycans (GAGs) as well as removing polysaccharides from a solution. The invention also includes methods of inhibiting anticoagulant activity, inhibiting angiogenesis, treating cancer, and inhibiting maternal malarial infection.

REFERENCES:
patent: 4341869 (1982-07-01), Langer et al.
patent: 5145778 (1992-09-01), Bellamy et al.
patent: 5169772 (1992-12-01), Zimmerman et al.
patent: 5198355 (1993-03-01), Kikuchi et al.
patent: 5198855 (1993-03-01), Iwai
patent: 5290695 (1994-03-01), Morikawa et al.
patent: 5338677 (1994-08-01), Zimmerman et al.
patent: 5389539 (1995-02-01), Sasisekharan et al.
patent: 5496718 (1996-03-01), Hashimoto et al.
patent: 5498536 (1996-03-01), Khandke
patent: 5525500 (1996-06-01), Khandke et al.
patent: 5567417 (1996-10-01), Sasisekharan et al.
patent: 5569600 (1996-10-01), Sasisekharan et al.
patent: 5578480 (1996-11-01), Khandke
patent: 5619421 (1997-04-01), Venkataraman et al.
patent: 5668274 (1997-09-01), Petitou et al.
patent: 5681733 (1997-10-01), Su et al.
patent: 5714376 (1998-02-01), Sasisekharan et al.
patent: 5716617 (1998-02-01), Khandke et al.
patent: 5741692 (1998-04-01), Khandke et al.
patent: 5763205 (1998-06-01), Hashimoto et al.
patent: 5773277 (1998-06-01), Hashimoto et al.
patent: 5801162 (1998-09-01), Takada et al.
patent: 5817645 (1998-10-01), Zamboni et al.
patent: 5830726 (1998-11-01), Sasisekharan et al.
patent: 5866120 (1999-02-01), Karageozian et al.
patent: 5919693 (1999-07-01), Su et al.
patent: 5965507 (1999-10-01), Thoen et al.
patent: 5968822 (1999-10-01), Pecker et al.
patent: 5997863 (1999-12-01), Zimmermann et al.
patent: 6001630 (1999-12-01), Ichikawa
patent: 6007810 (1999-12-01), Ishikawa et al.
patent: 6039943 (2000-03-01), Karageozian et al.
patent: 6054569 (2000-04-01), Bennett et al.
patent: 6063378 (2000-05-01), Nohara et al.
patent: 6093563 (2000-07-01), Bennett et al.
patent: 6184023 (2001-02-01), Hashimoto et al.
patent: 6190875 (2001-02-01), Ben-Artzi et al.
patent: 6217863 (2001-04-01), Godavarti et al.
patent: 6378527 (2002-04-01), Hungerford et al.
patent: 6597996 (2003-07-01), Venkatamaran et al.
patent: 6869789 (2005-03-01), Liu et al.
patent: 6962699 (2005-11-01), Pojasek et al.
patent: 2002/0122793 (2002-09-01), Liu et al.
patent: 2002/0128225 (2002-09-01), Liu et al.
patent: 2002/0169143 (2002-11-01), Sasisekharan et al.
patent: 2003/0008820 (2003-01-01), Kwan et al.
patent: 2003/0096281 (2003-05-01), Venkataraman et al.
patent: 2003/0099628 (2003-05-01), Liu et al.
patent: 2003/0191587 (2003-10-01), Venkataraman et al.
patent: 2003/0203385 (2003-10-01), Venkataraman et al.
patent: 2003/0219830 (2003-11-01), Venkataraman et al.
patent: 2004/0087543 (2004-05-01), Shriver et al.
patent: 2004/0091471 (2004-05-01), Myette et al.
patent: 2004/0091472 (2004-05-01), Pojasek et al.
patent: 2004/0092037 (2004-05-01), Sasisekharan et al.
patent: 2004/0147033 (2004-07-01), Shriver et al.
patent: 2004/0197933 (2004-10-01), Venkataraman et al.
patent: 2004/0204869 (2004-10-01), Venkataraman et al.
patent: 2004/0214228 (2004-10-01), Venkataraman et al.
patent: 2005/0037376 (2005-02-01), Sasisekharan et al.
patent: 2005/0065738 (2005-03-01), Raguram
patent: 2005/0214276 (2005-09-01), Myette et al.
patent: 2005/0227320 (2005-10-01), Pojasek et al.
patent: 2005/0233401 (2005-10-01), Liu et al.
patent: 2005/0233402 (2005-10-01), Liu et al.
patent: 2005/0233419 (2005-10-01), Pojasek et al.
patent: 2005/0266067 (2005-12-01), Sengupta et al.
patent: 2006/0024664 (2006-02-01), Sasisekharan et al.
patent: 0355831 (1990-02-01), None
patent: 0 613 949 (1994-09-01), None
patent: 1 607 478 (2005-12-01), None
patent: 54107586 (1979-08-01), None
patent: 2000044601 (2000-02-01), None
patent: WO 91/16070 (1991-10-01), None
patent: WO 93/08289 (1993-04-01), None
patent: WO 94/12618 (1994-06-01), None
patent: WO 95/13830 (1995-05-01), None
patent: WO 95/34635 (1995-12-01), None
patent: WO 96/01648 (1996-01-01), None
patent: WO 96/01894 (1996-01-01), None
patent: WO 97/16556 (1997-05-01), None
patent: WO 97/18835 (1997-05-01), None
patent: WO 99/58650 (1999-11-01), None
patent: WO 200139795 (2000-02-01), None
patent: WO 01/34781 (2001-05-01), None
patent: WO 01/35977 (2001-05-01), None
patent: WO 01/38399 (2001-05-01), None
patent: WO 01/53474 (2001-07-01), None
patent: WO 03/074080 (2003-09-01), None
patent: WO 2004/110360 (2004-12-01), None
patent: WO 2005/112986 (2005-12-01), None
Achur et al., “Characterization of Proteoglycans of Human Placenta and Identification of Unique Chondroitin Sulfate Proteoglycans of the Intervillous Spaces that Mediate the Adherence ofPlasmodium falciparum-infected Erythrocytes to the Placenta,”The Journal of Biological Chemistry, vol. 275(51), Dec. 22, 2000, pp. 40344-40356.
Alkhalil et al., “Structural Requirements for the Adherence ofPlasmodium falciparum-infected Erythrocytes to Chondroitin Sulfate Proteoglycans of Human Placenta,”The Journal of Biological Chemistry, vol. 275(51), Dec. 22, 2000, pp. 40357-40364.
Baker, J. R. et al., “Action Pattern and Substrate Specificity of the Hyaluronan Lyase from Group B Streptococci”,Biochem. J., vol. 348, 2000, pp. 465-471.
Catlow et al., “Hepatocyte Growth Factor/scatter Factor and its Interaction With Heparan Sulphate and Dermatan Sulphate”,Biochemical Society Transactions, 2003, vol. 31(Part 2), pp. 352-353.
Daidouji et al., “Neoplastic Changes in Saccharide Sequence of Dermatan Sulfate Chains Derived from Human Colon Cancer,”Digestive Diseases and Sciences, vol. 47(2) Feb. 2002, pp. 331-337.
Denholm et al., “Anti-Tumor Activities of Chondroitinase AC and Chondroitinase B: Inhibition of Angiogenesis, Proliferation and Invasion,”European Journal of Pharmacology, vol. 416, 2001, pp. 213-221.
Denholm et al., “Inhibition of Human Dermal Fibroblast Proliferation by Removal of Dermatan Sulfate,”European Journal of Pharmacology, vol. 400, 2000, pp. 145-153.
Dierks et al., “Posttranslational Formation of Formylglycine in Prokaryotic Sulfatases by Modification of Either Cysteine or Serine”,The Journal of Biological Chemistry, vol. 273(40), Oct. 2, 1998, pp. 25560-25564.
Ernst, S. et al., “Enzymatic Degradation of Glycosaminoglycans”Critical Reviews in Biochemistry and Molecular Biology, vol. 30(5), 1995, pp. 387-444.
Ernst, S. et al., “Direct Evidence for a Predominantly Exolytic Processive Mechanism for Depolymerization of Heparin-like Glycosaminoglycans by Heparinase I”,Proceedings of the National Academy of Sciences of the United States of America, vol. 95, Apr. 1998, pp. 4182-4187.
Gacesa et al., “Enzymic Degradation of Alginates”,International Journal of Biochemistry, vol. 24(4), 1992, pp. 545-552.
Gandra et al., “Anticoagulant Sulfated Glycosaminoglycans in the Tissues of the Primitive ChordateStyela plicata(Tunicata)”,Glycobiology, vol. 10(12), 2000, pp. 1333-1340.
Gerit et al., “Understanding the Rates of Certain Enzyme-Catalyzed Reactions: Proton Abstraction from Carbon Acids, Acyl-Transfer Reactions and Displacement Reactions of Phosphodiesters”,Biochemistry, vol. 32(45), Nov. 16, 2003, pp. 11943-11952.
Gu, K. et al., “Purification, Characterization and Specificity of Chondroitin Lyases and Glycuronidase fromFlavobacterium heparinum” Biochemistry Journal, vol. 312, 1995, pp. 569-577.
Habuchi, O., “Diversity and Functions of Glycosaminoglycan Sulfotransferases”,Biochimica Et Biophysica Acta, vol. 1474, 2000, pp. 115-127.
Homans, S. W., “A Molecular Mechanical Force Field for the Conformational Analysis of Oligosaccharides: Comparison of Theoretical and Crystal Structures of Manα1-3Manβ1-4GlcNAc”,Biochemistry, vol.

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