Discrete acyltransferases associated with type I polyketide...

Details Discrete acyltransferases associated with type I polyketide... Discrete acyltransferases associated with type I polyketide...

2006-12-26

2006-12-26

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

C435S069100, C435S193000

Reexamination Certificate

active

07153667

ABSTRACT:
Genetic and biochemical characterization of the leinamycin biosynthesis gene cluster fromStreptomyces atroolivaceusS-140 revealed two PKS genes, lnmI and lnmJ, that encode six PKS modules, none of which contains a cognate AT domain. The AT activity is provided in trans by a discrete protein, LnmG, which loads the malonyl coenzyme A extender unit onto the ACP domains of all six PKS modules. This finding provides a basis for methods of engineering modular polyketide synthases and polyketide synthase
onribosomal peptide synthetases.

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