Method for relative quantification of methylation of...

Details Method for relative quantification of methylation of... Method for relative quantification of methylation of...

2006-12-26

2006-12-26

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120

Reexamination Certificate

active

07153671

ABSTRACT:
A method is described for the relative quantification of the methylation of cytosine bases in DNA samples, wherein the following method steps are conducted:a) a genomic DNA sample is chemically converted with a reagent, wherein 5-methylcytosine and cytosine react differently and show a different base pairing behavior in the DNA duplex after the reaction;b) the DNA sample is amplified, whereby a fluorescently labeled dCTP or dGTP derivative is added;c) the amplified products are separated spatially from each other; andd) the fluorescence of the separated amplified products is measured quantitatively.

REFERENCES:
patent: 5786146 (1998-07-01), Herman et al.
patent: 5837832 (1998-11-01), Chee et al.
patent: 6046002 (2000-04-01), Davis et al.
patent: 6379889 (2002-04-01), Apffel et al.
patent: 4212939 (1993-10-01), None
patent: 19754482 (1999-07-01), None
patent: WO 9856952 (1998-12-01), None
Wang et al., Large-Scale Identification, Mapping and Genotyping of Single-Nucleotide Polymorphisms in the Human Genome, Science, May 1998, vol. 280, pp. 1077-1082.
Roche et al., “Capillary Electrophoresis in Biotechnology,” Biotechnology Progress, 1997, vol. 13, pp. 659-668.
Yurov et al., “High resolution multicolor fluorescence in situ hybridization using cyanine and fluorescein dyes: rapid chromosom identification by directly fluorescently labeled alphoid DNA probes,” 1996, vol. 97, pp. 390-398.
Olek et al., “A modified and improved method for bisulphite based cytosine methylation analysis,” Nucleic Acids Research, 24(24):5064-6 (1996).
Abstract of Paul et al., “Cytosine methylation: quantitation by automated genomic sequencing and GENESCAN analysis,” Biotechniques, 21(1):126-33 (1996).

Affiliated with

Also associated with

Rating

Method for relative quantification of methylation of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for relative quantification of methylation of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for relative quantification of methylation of... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3706843