Transcriptional factor enhancing the resistance of plants to...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers resistance to heat or cold

Reexamination Certificate

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C435S468000, C435S320100, C536S023100, C536S023600

Reexamination Certificate

active

07141720

ABSTRACT:
The present invention relates to a transcription factor of a plant, AtSIZ, induced by osmotic stress; a gene encoding the transcription factor; and a method for enhancing resistance to stress of a plant by using the gene. The AtSIZ protein isolated fromArabidopsis thalianais a polypeptide, which contains a C3H-type zinc finger motif and acts as a transcription regulation factor activating transcription of genes related to stress response of a plant. Therefore, a plant with the enhanced resistance to osmotic stress can be produced by over expressing the AtSIZ gene in the plant through transforming recombinant plasmid containing AtSIZ gene into a plant and consequently, the productivity of the plant can be increased.

REFERENCES:
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Guo et al. (PNAS, 101: 9205-9210, 2004).
Ming Li Wang, et al, “A Cluster of ABA-Regulated Genes onArabidopsis thalianaBAC T07M07,” Genome Research (4), pp. 325-333, Apr. 4, 1999.
Ingela Fridborg, et al, “TheArabidopsisDwarf MutantshiExhibits Reduced Gibberellin Responses Conferred by Overexpression of a New Putative Zinc Finger Protein,” The Plant Cell, vol. 11, pp. 1019-1031, Jun. 1999.
Zhongsen Li, et al, “PEI1. an Embryo-Specific Zinc Finger Protein Gene Required for Heart-Stage Embryo Formation inArabidopsis,” The Plant Cell, vol. 10, pp. 383-398, Mar. 1998.
Kazuko Yamaguchi-Shinozaki, et al, “Characterization of the expression of a desiccation-responsive rd29 gene ofArabidopsis thalianaand analysis of its promoter in transgenic plants,” Mol. Gen Genet, vol. 236, pp. 331-340, Jan. 1993.
Kjell-Ove Holmström, et al, “Production of theEscherichia colibetaine-aldehyde dehydrogenase, an enzyme required for the synthesis of the osmoprotectant glycine betaine, in transgenic plants,” The Plant Journal, 6(5), pp. 749-758, Nov. 1994.

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