Method for producing target substance using microorganisms...

Details Method for producing target substance using microorganisms... Method for producing target substance using microorganisms...

2006-08-29

2006-08-29

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

C435S106000, C435S115000, C435S108000

Reexamination Certificate

active

07097999

ABSTRACT:
The present invention describes a method for producing a target substance by utilizing a microorganism comprising culturing the microorganism in a medium, allowing the target substance to accumulate, and collecting the target substance from the medium. Also the microorganism used in the present invention is a mutant strain whereby maltose assimilation is controlled by the interaction between IIAGlcprotein of glucose PTS and MalK.

REFERENCES:
patent: 4346170 (1982-08-01), Sano et al.
Ehrmann et al. Identification of Endogenous Inducers of the mal Regulon in Escherichia coli. J. Bacteriol. (1987) 169(8): 3539-3545.
J. Monod, Growth, vol. 11, pp. 223-289, “The Phenomenon of Enzymatic Adaptation and its Bearings on Problems of Genetics and Cellular Differentiation”, 1947.
P. W. Postma, et al., Escherichia Coli and Salmonella Cellular and Molecular Biology, Second Edition, vol. 1, pp. 1149-1174, “Phosphoenolpyruvate Carbohydrate Phosphotransferase Systems” 1996.
D.A. Dean, et al., Journal of Biological Chemistry, vol. 265, No. 34, pp. 21005-21010, “Regulation of the Maltose Transport System ofEscherichia Coliby the Glucose-Specific Enzyme III of the Phosphoenolpyruvate-Sugar Phosphotransferase System”, Dec. 5, 1990.
S. Kuhnau, et al., Journal of Bacteriology, vol. 173, No. 7, pp. 2180-2186, “The Activities of theEscherichia ColiMalk Protein in Maltose Transport, Regulation, and Inducer Exclusion can be Seperated by Mutations”, Apr. 1991.
G. Q. Zeng, et al., Res. Microbiol., vol. 143, pp. 251-261, “Mutational Analysis of the Enzyme IIIGlcthe Phosphoenolpyruvate Phosphotransferase System inEscherichia Coli”, 1992.

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