Analytical element and method for determining a component in a t

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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422 56, 422 57, 435805, 436501, 436518, 436524, 436527, 436528, 436529, 436530, 436531, 436800, 436810, 436827, 436828, G01N 33543, G01N 33566

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048681067

ABSTRACT:
Disclosed are an analytical element for determining a specific component A in a test sample, based on the specific reaction between said specific component A and a substance B capable of binding specifically to said specific component A, by the use of a labelled material L comprising a label capable of providing a signal and said specific component A or comprising a label capable of providing a signal and a substance C capable of binding specifically to said specific component A, characterized in that said element has a porous reaction layer formed by the use of a mixture containing (a) a carrier having said substance B immobilized thereon and (b) a carrier having an absorbing substance D capable of binding specifically to said labelled material L which has not bound to said substance B or to said specific compound A, to thereby modulate said signal, and an analytical method employing the same.

REFERENCES:
patent: 4160645 (1979-07-01), Ullman
patent: 4161515 (1979-07-01), Ullman
patent: 4446232 (1984-05-01), Liotta
patent: 4447529 (1984-05-01), Greenquist et al.
patent: 4582792 (1986-04-01), Kasahara et al.
patent: 4657739 (1987-04-01), Yasuda et al.
International Union of Biochemistry, Enzyme Nomenclature, Academic Press, New York, 1979, pp. 201 and 278.

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