Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-12-05
2006-12-05
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200
Reexamination Certificate
active
07144702
ABSTRACT:
A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genus.The method for detecting species in a target plant genus, particularly an allergenic plant genus such as the genusFagopyrum,can make it possible to detect with high sensitivity, for example, about 1 ppm of the plant(s) in cases where the plant(s) is contained in a food ingredient or food product.
REFERENCES:
patent: 5876977 (1999-03-01), Wang et al.
patent: 5962665 (1999-10-01), Kroeger et al.
Lott et al., Nucleotide Sequence Analysis of the 5.8S rDNA and Adjacent ITS2 Region of Candida albicans and Related Species. Yeast (1993) 9:1199.
Holzhauser et al., Polymerase chain reaction (PCR) for detection of potentially allergenic hazelnut residues in complex food matrixes. Eur. Food Res. Technol. (2000) 211: 360-365.
Hartmann et al., Extensive Ribosomal DNA Genic Variation in the Columnar Cactus Lophocereus. Mol. Evol. (2001) 53: 124-134.
Lavin et al., The Dalbergioid Legumes (Fabeceae): Delimitation of a pantropical monophyletic clade. Amer. J. Bot. (2001) 88: 503-533.
Buck et al., Design Strategies and Performance of Custom DNA Sequencing Primers. BioTechniques (1999) 27: 528-536.
Jong Hee Shin et al., Rapid Identification of up to Three Candida Species in a Single Reaction Tube by a 5′ Exonuclease Assay Using Fluorescent DNA Probes, Journal of Clinical Microbiology, Jan. 1999, p. 165-170, vol. 37, No. 1 (Abstract).
Jana Proft et al., Identification of six sibling species of the Anopheles macilipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay, Parasitol Res (1999), 85: 837-843.
Michael Allmann et al., Polymerase chain reaction (PCR): a possible alternative to immunochemical methods assuring safety and quality of food; Z Lebensm Unters Forsch (1993) 196:248-251.
Hiramoto Masayuki
Hirao Takashi
Fredman Jeffrey
Hoffmann & Baron , LLP
House Foods Corporation
LandOfFree
Method for detecting target plant genus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detecting target plant genus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting target plant genus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3675462