Method for detecting protein

Details Method for detecting protein Method for detecting protein

2006-06-27

2006-06-27

Le, Long V. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C436S546000, C436S518000, C436S530000, C436S531000, C436S524000, C436S544000, C530S388900

Reexamination Certificate

active

07067270

ABSTRACT:
An object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.

REFERENCES:
patent: 4378428 (1983-03-01), Farina et al.
patent: 4469797 (1984-09-01), Albarella
patent: 5198537 (1993-03-01), Huber et al.
patent: WO 96/03423 (1996-02-01), None
Ed Harlow, et al., “Antibodies A Laboratory Manual,” Cold Spring Harbor Laboratory, pp. 53-138, 1988.

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