Computational method for designing enzymes for incorporation...

Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical

Reexamination Certificate

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C702S027000, C530S300000, C530S350000

Reexamination Certificate

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07139665

ABSTRACT:
The instant invention provides methods, reagents, and computational tools for designing non-natural substrate analogs for enzymes, especially for designing unnatural amino acid analogs for aminoacyl tRNA Synthetases (AARSs), such as the Phe tRNA Synthetase. The instant invention also provides methods to incorporate unnatural amino acid analogs, especially those with interesting functional groups, into protein products to generate proteins of modified or novel functions.

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Ohno et al (J Biochem (Tokyo), Sep. 2001; 130(3):417-23; see abstract.
Datta et al., A Designed Phenylalanyl-tRNA Synthetase Variant Allows Efficient in vivo Incorporation of Aryl Ketone Functionality into Proteins. Journal of the American Chemical Society, 124(20):5652-5653, 2002.
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Dahiyat and Mayo, Protein Design Automation.Protein Science5: 895-903, 1993.
IBBA et al., Relaxing the Substrate Specificity of an Aminoacyl-tRNA Synthetase Allows in vitro and in vivo Synthesis of Proteins Containing Unnatural Amino Acids. FEBS Letters 364: 272-275, 1994.
OHNO et al., Changing the amino acid specificity of yeast tyrosyl-tRNA synthetase by genetic engineering.J. Biochem(Tokyo). 130(3): 417-23, 2001. (Abstract).
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