Nucleic acid which is obtained from Tetrahymena and which...

Details Nucleic acid which is obtained from Tetrahymena and which... Nucleic acid which is obtained from Tetrahymena and which...

2006-11-14

2006-11-14

McElwain, Elizabeth F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

C800S013000, C800S281000, C800S021000, C435S320100, C536S023200

Reexamination Certificate

active

07135623

ABSTRACT:
The invention relates to nucleic acid(s) which is/are obtained from tetrahymena and which code(s) for a ciliate-specific delta-6-desaturase that is involved in the biosynthesis of commercially valuable, multiply unsaturated fatty acids (so-called PUFA) polyunsaturated fatty acids). The inventive nucleotide sequence and the polypeptide sequence that can be obtained therefrom exhibit a surprisingly low sequence identity compared to other known natural desaturases. The invention also relates to the use of the nucleic acid(s) for overexpression in ciliates, preferably tetrahymena, in particular,Tetrahymena thermophila,with the aim of increasing the production of delta-6 unsaturated fatty acids, especially GLA.

REFERENCES:
patent: 5614393 (1997-03-01), Thomas et al.
Broun et al, Science 282: 1315-1317, Nov. 13, 1998.
Van de Loo et al, PNAS, USA 92:6743-6747, Jul. 1995.
Doerks et al, TIG 14(6): 248-250, Jun. 1998.
Smith et al, Nature Biotechnology 15:1222-1223, Nov. 15, 1997.
Brenner, S.E., TIG 15(4): 132-133, Apr. 1999.
Bork et al, TIG 12(10): 425-427, Oct. 1996.
DeLuca, V., AgBiotech News and Information 5(6): 225N-229N, 1993.
Bertram, J., et al.: “Isolation of a Steroyl Coenzyme Desaturase From Tetrahymena Thermophila”, Journal of Protozoology, vol. 28, No. 1, 1981, pp. 127-131 XP002152567.
Koll, M., et al.: “The Effect of Dietary Sterol on the Activity of Fatty Acid Desaturases Isolated From Tetrahymena-Setosa”, Journal of Protozoology, vol. 37, No. 3, 1990, pp. 229-237, XP-002152568.
Fujiwara, Y., et al.: “Cytoplasmic Location of Linoleoyl-Coenzyme Desaturase in Microsomal Membranes of Rat Liver”, Archives of Biochemistry and Biophysics, vol. 233, No. 2, 1984, pp. 402-407, XP002152569.
Napier, et al.: Identification of Caenorhabditis Elegans Delta6-Fatty-Acid-Desaturase by Heterologos Expression in Saccharomyces Cerevisiae, Biochemical Journal, GB, Portland Press, London, vol. 330 No. 2, Mar. 1998, pp. 611-614, XP002099453.
Database Genbank Accession No. AF031477 May 2, 1998, “Caenorhabditis Elegans Delta6-Fatty-Acid-Desaturase mRNA, complete cds”.

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