Monkey-origin embryonic stem cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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C435S363000, C435S006120

Reexamination Certificate

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07083977

ABSTRACT:
A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

REFERENCES:
patent: 5843780 (1998-12-01), Thomson
patent: WO 96/22362 (1996-07-01), None
Thompson JA, Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts, 1996, Biology of Reproduction, vol. 55, pp. 254-259.
Hewitson L, Fertilization and embryo development to bastocyts after intracytoplasmic sperm injection in the rhesus monkey, 1998, Human Reproduction, vol. 13, pp. 3449-3455.
Sankai T, Cyryopreservation of spermatozoa from cynomolgus monkeys, 1994, J. of Reproduction and Fertility, vol. 101, pp. 273-278.
Hewitson L, Fertilization and embryo development to blastocyts after intracytoplasmic sperm injection in the rhesus monkey, 1998, Human Reproduction, vol. 13, pp. 3449-3455.
Thompson JA, Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts, 1996, Biology of Reproduction, vol. 55, pp. 254-259.
Thomson, James A., et al. Proceedings of the National Academy of Sciences of the USA, vol. 92, No. 17, pp. 7844-7848, Aug. 15, 1995.
Thomson, James A., et al. Current Topics in Developmental Biology, vol. 38, pp. 133-165, 1998.
Thomson, James A., et al. Common Marmoset (Callithrix jacchus) Blasocysts, Biology of Reproduction, vol. 55, No. 2, pp. 254-259, Aug. 1996.
Torii, Ryuzo, et al. Primates, vol. 41, No. 1, pp. 39-47, Jan. 2000.
Hewitson, L., et al. Human Reproduction, vol. 13, No. 12, pp. 3449-3455, Dec. 1998.
Miyoshi, Kazuyoshi, et al. Nippon Jusei Chakushou Gakkai Zasshi, Vo. 15, No. 1, pp. 180-182, Feb. 28, 1998.
Torii, R., et al. Theriogenology, vol. 55, No. 1, p. 374, Jan. 1, 2001.
Suemori Hirofumi et al., Developmental Dynamics, vol. 222, No. 2, Oct. 2001, pp. 273-279.

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