Method for producing L-amino acid

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S107000, C435S108000, C435S109000, C435S110000, C435S111000, C435S112000, C435S113000, C435S114000, C435S115000, C435S116000

Reexamination Certificate

active

07037690

ABSTRACT:
In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.

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J. M. Smith, et al., NCBI, GenBank Accession L20897, pp. 1-3, “Purine and One-Carbon Metabolism in ,Escherichia coliK12: DNA Sequence of a Second GAR Transformylase”, Jul. 26, 1993.
T. Conway, et al., NCBI, GenBank Accession X58364, pp. 1-2, “Cloning, Characterization and, Expression of theZymononas mobilisEDA Gene that Encodes 2-Keto-3-Deoxy-6-Phosphongluconate Aldolase of the Entner-Doudoroff Pathway”, Dec. 4, 1998.
W. O. Barnell, et al., NCBI, GenBank Accession M60615, M37982, pp. 1-4, “Sequence and Genetic Organization of aZymomonas mobilisGene Cluster that Encodes Several Enzymes of Glucose Metabolism”, Apr. 26, 1993.
N. Peekhaus, et al., Journal of Bacteriology, vol. 180, No. 14, pp. 3495-3502, XP-002262000, “What's for Dinner?: Entner-Doudoroff Metabolism inEscherichia coli”, Jul. 1998.
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