Polypeptides having polymerase activity and methods of use...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S015000, C435S194000, C435S183000

Reexamination Certificate

active

07056703

ABSTRACT:
The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived fromPyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.

REFERENCES:
patent: 4165188 (1979-08-01), Rempel
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5491086 (1996-02-01), Gelfand et al.
patent: H1531 (1996-05-01), Blumentals
patent: 5795763 (1998-08-01), Dahlberg et al.
patent: 5939250 (1999-08-01), Short
patent: 6008025 (1999-12-01), Komatsubara et al.
patent: 6077664 (2000-06-01), Slater et al.
patent: 6492511 (2002-12-01), Callen et al.
patent: 455430 (1991-04-01), None
patent: 0624641 (1994-11-01), None
patent: 07298879 (1995-11-01), None
patent: 07327684 (1995-12-01), None
patent: WO 99/07837 (1999-02-01), None
Ngo et al., Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox, in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.
Stetter et al., 1990, FEMS Microbiology Reviews 75:117-124.
Pley et al., 1991, Systematic and Applied Microbiology, 14:245.
Pearson et al., “Improved tools for biological sequence comparison”, Prc. Natl Acad. Sci USA, vol. 85, pp. 2444-2448, Apr. 1988 Biochemistry.
Madigan et al.,Scientific American, Extremophiles, Apr. 1997, pp. 82-87.
Choi et al., Purification and properties ofThermus filiformisDNA polymerase expressed inEscherichia coli, Biotechnol. Appl. Biochem.(1999) 30:19-25.
Uemori, et al., “The hyperthermophilic archaeon Pyrodictium occultum has two alpha-like DNA polymerases”,J. Bacteriology, vol. 177, No. 8, pp. 2164-2177, 1995.
Campbell, et al., “General properties and applications of monoclonal anitbodies”, Elsevier Science Publishers, secton 1.1. pp. 1-32, 1984.
Bost, et al., “Antibodies Against a Peptide Sequence Within the HIV Envelope Protein Crossreacts with Human Interleukin-2”,Immunological Investigations, vol. 17, No. 6 & 7, pp. 577-586, 1988.
Bendayan, “Possibilities of False Immunocytochemical Results Generated by the Use of Monoclonal Antibodies: The Example of the Anti-proinsulin Antibody”,The Jounral of Histochemistry and Cytochemistry, vol. 43, No. 9, pp. 881-886, 1995.
Colman, “Effects of amino acid sequence changes on antibody-antigen interactions”Research in Immunology, vol. 145, No. 1, pp. 33-36.
Lederman, et al., “A single Amino Acid Substitution in a Common African Allele of he CD4 Molecule Ablates Binding of the Monoclonal Antibody, OKT4”,Molecular Immnology, vol. 28, No. 11, pp. 1171-1181, 1991.
Abaza, et al., “Effects of Amino Acid Substitutions Outside an Antigenic Site on Protein Binding to Monoclonal Antibodies of predetermined Specificity Obtained by Peptide Immunization: Demonstration with Region 94-100 (Antigenic Site 3) of Myoglobin”,Journal of Protein Chemistry, vol. 11, No. 5, pp. 433-444.
Ngo, et al., “Computational Complexity, Protein Structure Prediction and the Levinthal Paradox”,The Protein Folding Problem, Ch. 14, pp. 491-494.
Deckert et al., Nature 392:353-358 (1998).
Edgell et al., Journal of Bacteriology 179:2632-2640 (1997).
Supplementary European Search Report mailed on Jul. 6, 2004, for European patent application No. 98940988, filed on Aug. 6, 1998, 4 pages.
Pisani et al., Nucleic Acids Research 20(11):2711-2716 (1992).

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