Transgenic animals expressing salivary proteins

Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal

Reexamination Certificate

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C800S004000, C800S008000, C800S017000, C800S024000

Reexamination Certificate

active

07115795

ABSTRACT:
The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a phytase or a homologue thereof. Such proteins may be heterologous and may be specifically expressed in the salivary gland of the animal by operably linking the nucleic acid sequence encoding the protein with regulatory sequence including a salivary gland protein promoter/enhancer. Also provided are methods of expressing and producing proteins using such nucleic acid constructs. Further, antibodies specific to such proteins and immunological diagnostic kits are also provided.

REFERENCES:
patent: 5837693 (1998-11-01), German et al.
patent: 5939303 (1999-08-01), Cheng
patent: 5985605 (1999-11-01), Cheng
patent: WO 97/48812 (1997-12-01), None
patent: WO 99/08539 (1999-02-01), None
patent: WO 99/17610 (1999-04-01), None
Hammer, 1990, Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human beta-2m : An animal model of HLA-B27-associated human disorders, Cell, vol. 63, pp. 1099-1112.
Mullins, 1990, Nature, Fulminant hypertension in transgenic rats harbouring the mose Ren-2 gene, vol. 344, pp. 541-544.
Sang, 2004, Mech. Dev., vol. 121, pp. 1179-1186.
Mozdziak, 2994, Dev. Dynam., vol. 229, pp. 414-421.
Higashijima, 1997, Dev. Biol., vol. 192, pp. 289-299.
Samuelson, 1996, Mol. Biol. Evol., vol. 13, pp. 767-779.
Saito, 1999, Jour. Immunol., vol. 162, pp. 2488-2494.
Velander (1992, PNAS, vol. 89, pp. 12003-12007.
Li, J et al., 1997, Plant Physiol., 114;1103-1111.
Hong, C-Y et al., 2004, Transgenic Research, 13:29-39.
Abelson, P.H. 1999. A potential phosphate crisis. Science 283: 2015.
Altschul,S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
Ann, D.K. and Carlson, D.M. 1985. The structure and organization of a proline-rich protein gene of a mouse multigene family. J. Biol. Chem. 260: 15863-15872.
AOAC 1984. Official Methods of analysis (14th Ed.).
Ausbel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. 1992. Short protocols in molecular biology. John Wiley & Sons.
Barsh, G.S. and Epstein, C.J. 1989. Physical and genetic characterization of a 75-kilobase deletion associated with al, a recessive lethal allele at the mouse agouti locus. Genetics 121: 811-818.
Bennick, A. 1982. Salivary proline-rich proteins. Mol.Cell Biochem. 45: 83-99.
Bitar, K. and Reinhold, J.G. 1972. Phytase and alkaline phosphatase activities in intestinal mucosae of rat, chicken, calf, and man. Biochim. Biophys. Acta 268: 442-452.
Burge, C. and Karlin S. 1997. Prediction of complete gene structures in human genomic DNA. J. Mol. Biol. 288: 78-94.
Burge, C.B. and Karlin, S. 1998. Finding the genes in genomic DNA. Curr. Opin. Struct. Biol. 8: 346-354.
Carlson, D.M. 1993. Salivary proline-rich proteins: Biochemistry, molecular biology, and regulation of expression. Crit. Rev.Oral Biol.Med. 4: 495-502.
Carswell, S. and Alwine, J.C. 1989. Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences. Mol. Cell. Biol. 9: 4248-4258.
Chi, T.H. and Crabtree, G.R. 2000. Inositol phosphates in the nucleus. Science 287: 1937-1939.
Clark, A.J., Archibald, A.L., McClenaghan, M., Simons, J.P., Wallace, R. and Whitelaw, C.B.A. 1993. Enhancing the efficiency of transgene expression. Phil.Trans.R.Soc.Lond. B 330: 225-232.
Clements, S., Mehansho, H. and Carlson, D.M. 1985. Novel multigene families encoding highly repetitive peptide sequences. J.Biol.Chem. 260: 13471-13477.
Corring,T. 1980. Endogenous secretions in the pig. In Current concepts of digestion and absorption in pigs. Edited by A.G.Low and I.G.Partridge. National Institute for Research in Dairying. Reading. pp.136-150.Ed.Technical Bulletin 3.
Cosgrove, D.J. 1980. Inositol phosphates. Their chemistry, biochemistry and physiology. Elsevier, Amsterdam.
Crameri, A., Dawes, G., Rodriguez, E.Jr., Silver, S. and Stemmer, W.P.C. 1997. Molecular evolution of an arsenate detoxification pathway by DNA shuffling. Nature Biotechnol. 15: 436-438.
Dassa, J., Marck, C. and Boquet, P.L. 1990. The complete nucleotide sequence of theEscheriichia coligeneappAreveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphatase. J. Bacteriol. 172: 5497-5500.
Denny, H.R. and Messervy, A. 1972. Surgical techniques for the extirpation of the submandibular salivary glands and the collection of salivary secretions in the pig. Vet.Rec. 90: 650-654.
Dobrinsky, J.R., Johnson, L.A. and Rath, D. 1996. Developmental of a culture medium (BECM-3) for porcine embryos: Effect of bovine serum albumin and fetal bovine serum on embryo development. Biol.Reprod. 55: 1069-1074.
Dvorakova, J. 1998. Phytase: Sources, preparation and exploitation. Folia Microbiol Prague. 43: 323-338.
Engelen, A.J., Vanderheeft, F.C., Randsdorp, P.H.G. and Smit, E.L.C. 1994. Simple and rapid determination of phytase activity, J.Aoac. Int. 77: 760-764.
Frandson, R.D. and Spurgeon, T.L. 1992. Anatomy and physiology of farm animals. Lea & Febiger, Philadelphia.
Galfre, G. and Milstein, C. 1981. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 73: 3-46.
Geyer, P.K. 1997. The role of insulator elements in defining domains of gene expression. Curr.Opin.Genet.Dev. 7: 242-248.
Gish, W. and States, D.J. 1993. Identification of protein coding regions by database similarity search. Nature Genetics. 3: 266-272.
Golovan, S., Wang, G., Zhang, J. and Forsberg, C.W. 2000. Characterization and overproduction of theEscherichia coliappA encoded bifunctional enzyme which exhibits both phytase and acid phosphatase activities. Can.J. Microbiol. 46: 59-71.
Gordon, J.W., Scangos, G.A., Plotkin, D.J., Barbosa, J.A. and Ruddle, F.H. 1980. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA 77: 7380-7384.
Gorman, C., Padmanabhan, R. and Howard, B.H. 1983. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553.
Greiner, R. and Jany, K.-D. 1991. Characterization of a phytase fromEscherichia coli. Herbsttagung der Gesellschaft fur Biologische Chemie 372 (Abstract).
Greiner, R., Konietzny, U. and Jany, K. 1993. Purification and characterization of two phytases fromEscherichia coli. Arch.Biochem.Biophys. 303: 107-113.
Hall, J., Hazelwood, G.P., Surani, M.A., Hirst, B.H. and Gilbert, H.J. 1990. Eukaryotic and prokaryotic signal peptides direct secretion of a bacterial endoglucanase by mammalian cells. J. Biol. Chem. 275: 19996-19999.
Harayama, S. 1998. Artificial evolution by DNA shuffling. Trends Biotechnol. 16: 76-81.
Harland, B.F. and Morris, E.R. 1995. Phytate: A good or a bad food component? Nutr.Res. 15: 733-754.
Harlow, E. and Lane, D. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, New York.
Heinoen, J.K. and Lahti, R.J. 1981. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphate. Anal. Biochem. 133: 313-317.
Heneine, W. and Switzer, W.M. 1996. Highly sensitive and specific polymerase chain reaction assays for detection of baboon and pig cells following xenotransplantation in humans. Transplantation 62: 1360-1362.
Higuchi, R. 1989. Simple and rapid preparation of samples for PCR. In PCR technology: principles and applications for DNA amplification. Edited by H.A. Erlich. MacMillan Publishers; Stockton Press. New York. pp. 31-38.
Hu, Y., Nakagwa, Y., Purushotham, K.R. and Humphreys-Beher, G. 1992. Functional changes in salivary glands of autoimmune disease-prone NOD mice. Am. J. Physiol. 263: E607-E614.
Huang, X. 1996. An improved sequence assembly program. Genomics 33: 21-31.
Huang, X. 1999. A contig assembly program based on sensitive detection of fragment overlaps. Genomics 14: 18-25.
Huff, W.E., Moore, P.A.J. Waldroup, P.W., Waldroup, J.M., Balog, J.M., Huff, G.R., Rath, N.C., Daniel, T.C. and Raboy, V. 1998. Effect of dietary phytase and high avai

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