Nucleic acid sequencing with solid phase capturable terminators

Details Nucleic acid sequencing with solid phase capturable terminators Nucleic acid sequencing with solid phase capturable terminators

1998-09-16

2000-04-04

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 231, 536 253, C12Q 168, C12P 1934, C07H 2104

Patent

active

060460059

ABSTRACT:
Methods of enzymatic nucleic acid sequencing are provided in which solid-phase capturable chain terminators are employed. In the subject methods, sequencing fragments are generated, where the fragments comprise capturable chain terminators. The fragments are then captured on a solid phase and separated from the remaining components of the sequencing reaction. The fragments are then released from the solid phase, size separated and detected to yield sequencing data from which the sequence of the nucleic acid is determined.

REFERENCES:
patent: 4772691 (1988-09-01), Herman
patent: 4888274 (1989-12-01), Radding et al.
patent: 5393877 (1995-02-01), McLean et al.
patent: 5401632 (1995-03-01), Wang et al.
patent: 5405746 (1995-04-01), Uhlen
patent: 5484701 (1996-01-01), Cocuzza et al.
patent: 5518900 (1996-05-01), Nikiforov et al.
patent: 5573913 (1996-11-01), Rosemeyer et al.
patent: 5610287 (1997-03-01), Nikiforov et al.
Ansorge, W., et al., (1987) "Automated DNA Sequencing: Ultrasensitive Detection of Fluorescent Bands During Electrophoresis," Nucl. Acids Res., vol. 15, (11): 4593-4602.
Beck, S., et al., (1989) "Chemiluminescent Detection of DNA: Application for DNA Sequencing and Hybridization," Nucl. Acids Res. vol. 17(13):5115-5123.
Beck, S., (1990) "Colorimetric-Detected DNA Sequencing," Meth. in Enzymology vol. 184: 612-617.
Griffin, H.G., et al., (1993) "DNA Sequencing--Recent Innovations and Future Trends," Appl. Biochem. and Biotech. vol. 38:147-159.
Ju, J. et al. (1996) "Energy Transfer Primers: A New Fluorescence Labeling Paradigm for DNA Sequencing and Analysis," Nature Medicine, vol. 2(2): 246-249.
Livak, K.J., et al., (1992) "Detection of Single Base Differences Using Biotinylated Nucleotides with Very Long Linker Arms," Nucl. Acid Res. vol. 20(18): 4831-4837.
McGuigan, et al., (1993) "DNA Fingerprinting by Sampled Sequencing," Methods in Enzymology vol. 218:241-257.
Perkin Elmer (1990) "Guide to Interpretation of 373A Dye Primer and Dye Terminator Data," User Bulletin Model 373A DNA Sequencing System, vol. 17:1-6.
Richterich, P., (1989) "Non-Radioactive Chemical Sequencing of Biotin Labelled DNA," Nucl. Acids Res., vol. 17:(6):2181-2186.
Roskey, M.T., et al., (1996) "DNA Sequencing by Delayed Extraction-Matrix-Assisted Laser Desorption/Ionization Time of Light Mass Spectrometry," Proc. Natl. Acad. Sci. USA vol. 93: 4724-4729.
Seliger, H., et al., (1990) "New Methods for the Solid-Phase Sequence Analysis of Nucleic Acid Fragments Using the Sanger Dideoxy Procedure," Nucleosides & Nucleotides, vol. 9 (3): 383-388.
Stahl, S., et al., (1988) "Solid Phase DNA Sequencing Using the Biotin-Avidin System," Nucl. Acids Res. vol. 16(7): 3025-3038.
Tong, X., et al., (1992) "Solid-Phase Method for the Purification of DNA Sequencing Reactions," Anal. Chem. vol. 64:2672-2677.
Tong, X., et al., (1993), "Solid Phase Purification in Automated DNA Sequencing," DNA Sequence-J.DNA Sequencing and Mapping, vol. 4, 151-162.
Wahlberg, J., et al., (1990), "General Colorimetric Method for DNA Diagnostics Allowing Direct Solid-Phase Genomic Sequencing of the Positive Samples," Proc. Natl. Acad. Sci. USA vol. 87:6569-6573.
Wahlberg, J., et al., (1990) "Rapid Detection and Sequencing of Specific In Vitro Amplified DNA Sequences Using Solid Phase Methods," Mol. and Cell. Probes vol. 4: 285-297.
Wahlberg, J., et al., (1992), "Automated Magnetic Preparation of DNA Templates for Solid Phase Sequencing," Electrophoresis vol. 13:547-551.

Affiliated with

Also associated with

Rating

Nucleic acid sequencing with solid phase capturable terminators does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid sequencing with solid phase capturable terminators , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid sequencing with solid phase capturable terminators will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-363928