Process for preparing lysozyme

Details Process for preparing lysozyme Process for preparing lysozyme

2006-06-13

2006-06-13

Naff, David M. (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S183000, C435S188000, C435S815000, C435S816000

Reexamination Certificate

active

07060478

ABSTRACT:
A process for preparing lysozyme is provided which is characterized by mixing the egg white or its diluted solution with diatomaceous earth, kaolin, zeolite, or the mixtures thereof, and followed by eluting the adsorbed lysozyme with a salt solution. The diatomaceous earth, kaolin, and zeolite can specifically adsorb the lysozyme in egg white, and the adsorbed lysozyme can be easily eluted with a salt solution. According to the process of the present invention, the lysozyme in egg white can be easily and effectively isolated.

REFERENCES:
patent: 3767531 (1973-10-01), Olson et al.
patent: 3909358 (1975-09-01), Stanley et al.
patent: 4532212 (1985-07-01), Odell
patent: 4839419 (1989-06-01), Kraemer et al.
patent: 5075430 (1991-12-01), Little
Alderton et al., Isolation of lysozyme from egg white, J. Biol. Chem., 1945, pp. 43-57.

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