Primate embryonic stem cells

Details Primate embryonic stem cells Primate embryonic stem cells

2006-04-18

2006-04-18

Woitach, Joseph (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

C435S366000, C435S373000

Reexamination Certificate

active

07029913

ABSTRACT:
A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

REFERENCES:
patent: 5541081 (1996-07-01), Hardy et al.
patent: 5843780 (1998-12-01), Thomson
patent: 6200806 (2001-03-01), Thomson

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