Method of screening anti-mycobacterial molecules

Details Method of screening anti-mycobacterial molecules Method of screening anti-mycobacterial molecules

2006-06-27

2006-06-27

Swartz, Rodney P (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Recombinant or stably-transformed bacterium encoding one or...

C424S009100, C424S009200, C424S130100, C424S164100, C424S168100, C424S185100, C424S192100, C424S199100, C424S234100, C424S248100, C435S004000, C435S029000, C435S032000, C435S183000, C435S253100

Reexamination Certificate

active

07067136

ABSTRACT:
This invention relates to a novel mycobacterial protein named DES, which appears to share significant amino acid sequence homology with soluble stearoyl-ACP desaturases. The results of allelic exchange experiments, indicate that the des gene may be essential to the survival of mycobacteria. These results coupled with the surface localization, the unique structure of DES, and the fact this antigen is expressed in vivo, and DES protein induces a humoral response in human patients, indicate that the DES protein provides a new target for the design of anti-mycobacterial drugs. This invention provides methods of screening molecules that can inhibit the DES enzyme activity of purified DES protein, in order to identify antibiotic molecules that are capable of inhibiting the growth or survival of mycobacteria. These methods may be practiced by using recombinant DES protein obtained from a recombinantmycobacteriumhost cell that was transformed with a vector containing the des gene, whose expression is controlled by regulatory or promoter sequences that function in mycobacteria. Another aspect of this invention relates to the molecules that have been identified according to the screening methods as having antibiotic activity, against mycobacteria.

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