Method and equipment to monitor nucleic acid hybridization...

Details Method and equipment to monitor nucleic acid hybridization... Method and equipment to monitor nucleic acid hybridization...

2006-09-05

2006-09-05

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300, C422S068100

Reexamination Certificate

active

07101671

ABSTRACT:
This invention is to provide a method for detecting DNA hybridization on DNA chips using 4-D parameters. Based on the traditional method of using 3-D parameters to detect DNA hybridization on DNA chips, this invention introduces temperature as the forth parameters to determine the melting temperatures by scanning the changes of fluorescence intensity of the fluorescent labels on the double-stranded oligo nuclei acids resulted from the increasing of hybridization temperature. Comparing the measured melting temperatures with the standard melting temperature specific for each probe on the chip is used to gain insight into the specific property of the single-stranded nucleic acids in the sample. This device is composed of a DNA chip containing transparent glass chamber, in which a temperature sensor and a thermo-circler are installed. This invention provides a specific, sensitive, and easy operating method to detect gene in a complex sample. The device used in this invention has simple structure, and can be coupled with various types of DNA chips with low cost.

REFERENCES:
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5744305 (1998-04-01), Fodor et al.
patent: 6238686 (2001-05-01), Carrino et al.
patent: 2001/0000148 (2001-04-01), Kurane et al.
patent: 1252453 (2000-05-01), None
patent: 1284568 (2001-02-01), None

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