Multiplex PCR for the detection of AmpC beta-lactamase genes

Details Multiplex PCR for the detection of AmpC beta-lactamase genes Multiplex PCR for the detection of AmpC beta-lactamase genes

2006-05-16

2006-05-16

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S024330

Reexamination Certificate

active

07045291

ABSTRACT:
Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain AmpC beta-lactamases. The primers can be employed in methods to detect the presence or absence of an AmpC beta-lactamase gene in samples, and to identify nucleic acid characteristic of AmpC beta-lactamase genes in samples, particularly, in clinical isolates of Gram-negative bacteria.

REFERENCES:
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5840549 (1998-11-01), First et al.
patent: 5994066 (1999-11-01), Bergeron et al.
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6210885 (2001-04-01), Gjerde et al.
patent: 6238868 (2001-05-01), Carrino et al.
patent: 6242223 (2001-06-01), Hanson et al.
patent: 6303288 (2001-10-01), Furcht et al.
patent: 6309833 (2001-10-01), Edman et al.
patent: 6379889 (2002-04-01), Apffel et al.
patent: WO 91/08305 (1991-06-01), None
patent: WO 2001/23604 (2001-04-01), None
patent: WO 2001/23604 (2001-04-01), None
patent: WO 02/086153 (2002-10-01), None
“Designer PCR™” from Research Genetics (2130 Memorial Parkway SW, Huntsville, AL). Advertisement inNucleic Acis Res., 22(15) 1994.
Barnaud et al., “Extension of Resistance to Cefepime and Cefpirome Associated to a Six Amino Acid Deletion in the H-10 Helix of the Cephalosporinase of anEnterobacter CloacaeClinical Isolate,”FEMS Microbiology Letters, 195(2):185-190 (2001).
Galleni et al., “Sequence and comparative analysis of threeEnterobacter cloacae ampC β-lactamase genes and their products,”Biochem. J., 250,753-760 (1988).
Gonzalez Leiza et al., “Gene sequence and biochemical characterization of FOX-1 fromKlebsiella pneumoniae,a newe AmpC-type plasmid-mediated beta-lactamase with two molecular variants,”Antimicrob. Agents Chemother., 38(9):2150-7 (1994).
Huletsky et al., “Nucleotide Sequence and Phylogeny of SHV-2 β-Lactamase,”Antimicrob. Agents Chemother., 34(9):1725-1732 (1990).
Leung et al., “Rarity of transferable β-lactamase production byKlebsiellaspecies,”J. Antimicrob. Chemother., 39:737-745 (1997).
Martineau et al., “Species-specific and ubiquitous DNA-based assays for rapid identification ofStaphylococcus epidermidis,” J. Clin. Microbiol., 34(12):2888-93 (1996).
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD. GenBank accession No. AY034848:Klebsiella pneumoniae :<http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=nucleotide&list—uids=14626421&dopt=GenBank> (retrieved on Sep. 27, 2002).
Transgenomic |Products and Technology; “Nucleic Acid Separations—WAVE® Nucleic Acid Fragment Analysis System Technology—Instruments & Accessories” <http://www.transgenomic.com/Pages/ProductWave.html> (retrieved on Nov. 8, 2001).
Transgenomic |Products and Technology; “WAVE® System Technologies” <http://www.transgenomic.com/Pages/ProductWave.html> (retrieved on Feb. 22, 2002).
Acar et al., “Nature of the Resistance Problem,”Clin. Inf. Dis., 24(Suppl 1):S1 (1997).
Arlet et al., “Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable β-lactamases (TEM, SHV, CARB),”FEMS Microbiol. Lett., 82:19-25 (1991).
Arlet et al., “Molecular characterization by PCR-restriction fragment length polymorphism of TEM β-lactamases,”FEMS Microbiol. Lett., 134:203-208 (1995).
Arlet et al., “Substitution of alanine for aspartate at position 179 in the SHV-6 extended-spectrum β-lactamases,”FEMS Microbiol. Lett., 152:163-167 (1997).
Barnaud et al., “Cloning and sequencing of the gene encoding the AmpC β-lactamase ofMorganella morganii,” FEMS Microbiol. Lett, 148:15-20 (1997).
Bauernfeind et al., “A New Plasmidic Cefotaximase in a Clinical Isolate ofEscherichia coli,” Infection, 18(5):294-298 (1990).
Billot-Klein et al., “Nucleotide Sequence of the SHV-5 β-Lactamase Gene of aKlebsiella pneumoniaePlasmid,”Antimicrob. Agents Chemother., 34(12):2439-2441 (1990).
Birnboim et al., “A rapid alkaline extraction procedure for screening recombinant plasmid DNA,”Nucleic Acids Res., 7(6):1513-1523 (1979).
Bradford et al., “SHV-7, a Novel Cefotaxime-Hydrolyzing β-Lactamase, Identified inEscherichia coliIsolates from Hospitalized Nursing Home Patients,”Antimicrob. Agents Chemother., 39(4):899-905 (1995).
Bradford et al., “Multiply ResistantKlebsiella pneumoniaeStrains from Two Chicago Hospitals: Identification of the Extended-Spectrum TEM-12 and TEM-10 Ceftazidime-Hydrolyzing β-Lactamases in a Single Isolate,”Antimicrob. Agents Chemother., 38(4): 761-766 (1994).
Bret et al., “Chromosomally Encoded AmpC-Type β-Lactamase in a Clinical Isolate ofProteus mirabilis,” Antimicrob. Agents Chemother., 42(5):1110-1114 (1998).
Brun-Buisson et al., “Transferable Enzymatic Resistance to Third-Generation Cephalosporins During Nosocomial Outbreak of MultiresistantKlebsiella Pneumoniae,” The Lancet, 2:302-306 (1987).
Burns et al., “An Integrated Nanoliter DNA Analysis Device,”Science, 282:484-487 (1998).
Caniça et al., “Molecular Diversity and Evolution ofblatemGenes Encoding β-Lactamases Resistant to Clavulanic Acid in ClinicalE. coli,” J. Mol. Evol., 44:57-65 (1997).
Carter et al., “Use of a non-radioactive hybridisation assay for direct detection of gram-negative bacteria carrying TEM β-lactamase genes in infected urine,”J. Med. Microbiol, 28:113-117 (1989).
Check, “Clinical Microbiology Eyes Nucleic Acid-Based Technologies,”ASM News, 64(2):84-89 (1998).
Crea et al., “Chemical synthesis of genes for human insulin,”Proc. Natl. Acad. Sci. USA, 75(12):5765-5769 (1978).
Crosa et al., “Plasmids,”Manual of Methods for General Bacteriology,Gerhardt et al., eds., American Society for Microbiology, Washington, DC, Ch.15, pp. 266-282 (1981).
Curran et al., “A rapid immunoassay method for the direct detection of PCR products: application to detection of TEM β-lactamase genes,”J. Med. Microbiol., 45:76-78 (1996).
Gold et al., “Antimicrobial-Drug Resistance,”The New England Journal of Medicine, 335(19):1445-1453 (1996).
Hanson et al., “Molecular Characterization of a Multiply ResistantKlebsiella pneumoniae,”Abstract C-59,37thICAAC,Toronto, Ontario, Canada, Sep. 28-Oct. 1, (1997).
Hanson et al., “A Novel TEM-Type Extended Spectrum Beta-Lactamase Expressed in Three Different Genera of Enterobacteriaceae from South Africa,” Abstract C-5, p. 70,38thICAAC,San Diego, California, Sep. 24-27 (1998).
Hanson et al., “Molecular characterization of a multiply resistantKlebsiella pnuemoniaeencoding ESBLs and a plasmid-mediated AmpC,”J. Antimicrob. Chemother., 44:377-380 (1999).
Hanson et al., “Regulation of Inducible AmpC Beta-Lactamase Expression AmongEnterobacteriaceae,” Curr. Pharmac. Design, 5(11):881-894 (1999).
Hibbert-Rogers et al., “Convergent evolution of TEM-26, a β-lactamase with extended-spectrum activity,”J. Antimicrob. Chemother., 33:707-720 (1994).
Jacoby et al., “More Extended-Spectrum β-Lactamases,”Antimicrob. Agents Chemother., 35(9):1697-1704 (1991).
Jarlier et al., “Extended Broad-Spectrum β-Lactamases Conferring Transferable Resistance to Newer β-Lactam Agents inEnterobacteriaceae:Hospital Prevalence and Susceptibility Patterns,”Rev. Infect. Dis., 10(4):867-878 (1988).
Jones, “The Emergent Needs for Basic Research, Education, and Surveillance of Antimicrobial Resistance: Problems Facing the Report from the American Society for Microbiology Task Force on Antibiotic Resistance,”Diagn. Mi

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