Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-09-05
2006-09-05
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C435S091100
Reexamination Certificate
active
07101673
ABSTRACT:
A method for detecting amplified nucleic acid fragments is provided. The method comprises the steps of carrying out amplification reaction to amplify a predetermined region of a nucleic acid in a reaction solution comprising the nucleic acid as a template, a pair of primers, deoxynucleotides, and dideoxynucleotides; hybridizing the amplified products of the reaction solution to a probe; and then detecting the hybridization between the amplified products and the probe.
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patent: 6858412 (2005-02-01), Willis et al.
Ruano et al. (“Coupled amplification and sequencing of genomic DNA” Proc. Natl. Acad. Sci. Apr. 1991. vol. 88: pp. 2815-2819).
Valentine et al. (“Detection of Helibacter pylori by Using the Polymerase Chain Reaction” Journal of Clinical Microbiology. Apr. 1991. vol. 29, No. 4: pp. 689-695).
Deng et al. “Simulttaneous amplification and sequencing of genomic DNA (SAS): sequencing of 16S rRNA genes using total genomic DNA fromButyrivibrio fibrisolvens, and detection and genotyping of nonculturable mycoplamsa-like organisms directly from total DNA isolated from infected plants” Journal of Microbiological Methods. 1993. 17: pp. 103-113.
Ito Seiichiro
Tanoue Junko
Yasukawa Fumiko
A. Marquez, Esq. Juan Carlos
Babic Christopher M
Fisher Esq. Stanley P.
Hitachi Software Engineering Co. Ltd.
Horlick Kenneth R.
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