Methods for preparing yeast with improved biotin...

Details Methods for preparing yeast with improved biotin... Methods for preparing yeast with improved biotin...

2006-04-25

2006-04-25

Kerr, Kathleen M. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S252200, C435S252200, C435S477000, C435S483000

Reexamination Certificate

active

07033814

ABSTRACT:
Methods for preparing yeast with improved biotin productivity using integrating plasmids encoding biotin synthase. The yeast is transformed by an integrating plasmid, which includes aCandida utilisbiotin synthase gene BIO2, an assistant DNA sequence to promote integration of the plasmid into theC. utilisgenome, a promoter sequence, and a selection marker. Other embodiments includeSaccharomyces cerevisiaeintegrating plasmids.

REFERENCES:
patent: 2216530 (1989-10-01), None
Steams et al. Manipulating yeast genome using plasmid vectors. Methods in Enzymology (1990) 185: 280-297.
Zhang et al. The gene for biotin synthase fromSaccharomyces cerevisiae: cloning, sequencing, and complementation ofEscherichia colistrains lacking biotin synthase. Arch Biochem Biophys (1994) 309(1): 29-35.
Hong et al. Candida utilis biotin synthase (Bio2) gene, complete cds. GenBank Accession No. AF212161, publicly available Dec. 7, 2000.
Rodriguez et al. Development of an integrative DNA transformation system for the yeast Candida utilis. FEMS Microbiol Lett (1998) 165(2): 335-340.
K.Kondo, et al.; A Transformation System for the YeastCandida utilis:Use of a Modified Endogenous Ribosomal Protein Gene as a Drug-resistant Marker and Ribosomal DNA as an Integration Target for Vector DNA; Journal of Bacteriology, Dec. 1995; pp. 7171-7177.
P.Dehoux, et al.; Natural Cycloheximide Resistance in Yeast The Role of Ribosomal Protein L41; Eur.J.Biochem. 213, 1993 pp. 841-848.
S. Kawai, et al.; Drastic Alteration of Cycloheximide Sensitivity by Substitution of One Amino Acid in the L41 Ribosomal Protein of Yeasts; Journal of Bacteriology, Jan. 1992; pp. 254-262.

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