Oligonucleotide primers having SEQ ID NOs. 1 to 21 and a...

Details Oligonucleotide primers having SEQ ID NOs. 1 to 21 and a... Oligonucleotide primers having SEQ ID NOs. 1 to 21 and a...

2006-05-09

2006-05-09

Navarro, Mark (Department: 1645)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

Reexamination Certificate

active

07041482

ABSTRACT:
The present invention relates to oligonucleotide primers having SEQ ID NOs. 1 to 21 specific forSalmonellaenterotoxin gene (stn) gene, useful for rapid and specific screening ofSalmonella. The present invention relates to a process for the rapid and specific detection ofSalmonellaenterotoxin gene (stn) gene in a subject for the presence of parasiteSalmonellausing oligonucleotide primers having SEQ ID NOs. 1 to 21 for Polymerase Chain Reaction (PCR), said process comprising the steps of preparing a DNA template, amplifying the template using the primers by PCR, running the PCR products on gel, and detecting theSalmonella.

REFERENCES:
Carli et al. (2001) “Detection ofSalmonellaein chicken feces by a combination of tetrathionate broth enrichment, capillary PCR, and capillary gel electrophoresis”,Journal of Clinical Microbiology, 39: 1871-1876.
Ferretti et al. (2001) “Twelve-hour PCR-based method for detection ofSalmonellaspp. in food”,Applied and Environmental Microbiology, 67: 977-978.
Mead et al. (1999) “Food-related illness and death in the United States”,Emerging Infectious Diseases, 5: 607-625.

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