Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-07-25
2006-07-25
Chernyshev, Olga N. (Department: 1649)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S320100, C435S366000, C435S252100, C435S252300
Reexamination Certificate
active
07081337
ABSTRACT:
The present invention is directed to isolated nucleic acids encoding Mint protein variants having enhanced abilities to modulate the transcriptional activation mediated by the cytoplasmic tail of the amyloid precursor protein (APP) relative to wild-type Mint proteins. The present invention is further directed toward purified Mint protein variants having enhanced abilities to modulate the transcriptional activation mediated by the cytoplasmic tail of APP relative to wild-type Mint proteins. The present invention also encompasses methods of modulating transcriptional activation and methods of identifying compounds that modulate transcriptional activation, and vectors, as well as transfected cells and kits useful for modulating transcriptional activation or for the identification of compounds that can modulate transcriptional activation. The present invention further encompasses transgenic knockout mice with little or no expression of Mint 1, Mint 2 or Mint 3 proteins. Such reagents may be useful as candidate therapeutics for Alzheimer's disease (AD), or as models for the rational design of drugs useful for the treatment of AD.
REFERENCES:
patent: 2001/0034884 (2001-10-01), Peraus
Mori A, Okuyama K, Horie M, Taniguchi Y, Wadatsu T, Nishino N, Shimada Y, Miyazawa N, Takeda S, Niimi M, Kyushiki H, Kondo M, Mitsumoto Y. Alteration of methamphetamine-induced striatal dopamine release in mint-1 knockout mice. Neurosci Res Jul. 2002;43(3):251-257.
Biederer T, Sudhof TC. CASK and protein 4.1 support F-actin nucleation on neurexins. J Biol Chem Dec. 21, 2001;276(51):47869-47876.
Cao X, Sudhof TC. A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60. Science Jul. 6, 2001;293(5527):115-120.
Cupers P, Orlans I, Craessaerts K, Annaert W, De Strooper B. The Amyloid precursor protein (APP)-cytoplasmic fragment generated by gamma-secretase is rapidly degraded but distributes partially in a nuclear fraction of neurones in culture. J Neurochem Sep. 2001;78(5):1168-1178.
Gao Y, Pimplikar SW. The gamma -secretase-cleaved C-terminal fragment of amyloid precursor protein mediates signaling to the nucleus. Proc Natl Acad Sci U S A Dec. 18, 2001;98(26):14979-14984.
Kimberly WT, Zheng JB, Guenette Sy, Selkoe DJ. The intracellular domain of the beta-amyloid precursor protein is stabilized by Fe65 and translocates to the nucleus in a notch-like manner. J Biol Chem Oct. 26, 2001;276(43):40288-40292.
Sastre M, Steiner H, Fuchs K, Capell A, Multhaup G, Condron MM, Teplow DB, Haass C. Presenilin-dependent gamma-secretase processing of beta-amyloid precursor protein at a site corresponding to the S3 cleavage of Notch. EMBO Rep Sep. 2001;2(9):835-841.
Wolfe MS, Haass C. The Role of presenilins in gamma-secretase activity. J Biol Chem Feb. 23, 2001;276(8):5413-5416.
Yu C, Kim SH, Ikeuchi T, Xu H, Gasparini L, Wang R, Sisodia SS. Characterization of a presenilin-mediated amyloid precursor protein carboxyl-terminal fragment gamma. Evidence for distinct mechanisms involved in gamma -secretase processing of the APP and Notch1 transmembrane domains. J Biol Chem Nov. 23, 2001;276(47):43756-43760.
Biederer T, Sudhof TC. Mints as adaptors. Direct binding to neurexins and recruitment of munc18. J Biol Chem Dec. 22, 2000;275(51):39803-39806.
Brown MS, Ye J, Rawson RB, Goldstein JL. Regulated intramembrane proteolysis: a control mechanism conserved from bacteria to humans. Cell Feb. 18, 2000;100(4):391-398.
Esler WP, Kimberly WT, Ostaszewski BL, Diehl TS, Moore CL, Tsai JY, Rahmati T, Xia W, Selkoe DJ, Wolfe MS. Transition-state analogue inhibitors of gamma-secretase bind directly to presenilin-1. Nat Cell Biol Jul. 2000;2(7):428-434.
Lau KF, McLoughlin DM, Standen C, Miller CC. XII alpha and x11 beta interact with presenilin-1 via their PDZ domains. Mol Cell Neurosci Nov. 2000;16(5):557-565.
Mueller HT, Borg JP, Margolis B, Turner RS. Modulation of amyloid precursor protein metabolism by XIIalpha /Mint-1. A deletion analysis of protein-protein interaction domains. J Biol Chem Dec. 15, 2000;275(50):39302-39306.
Okamoto M, Matsuyama T, Sugita M. Ultrastructural localization of mint1 at synapses in mouse hippocampus. Eur J Neurosci Aug. 2000;12(8):3067-3072.
Setou M, Nakagawa T, Seog DH, Hirokawa N. Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science Jun. 9, 2000;288(5472):1796-1802.
Struhl G, Adachi A. Requirements for presenilin-dependent cleavage of notch and other transmembrane proteins. Mol Cell Sep. 2000;6(3):625-636.
Verhage M, Maia AS, Plomp JJ, Brussaard AB, Heeroma JH, Vermeer H, Toonen RF, Hammer RE, van den Berg TK, Missler M, Geuze HJ, Sudhof TC. Synaptic assembly of the brain in the absence of neurotransmitter secretion. Science Feb. 4, 2000;287(5454):864-869.
Bayer TA, Cappai R, Masters CL, Beyreuther K, Multhaup G. It all sticks together—the APP-related family of proteins and Alzheimer's disease. Mol Psychiatry Nov. 1999;4(6):524-528.
Borg JP, Lopez-Figueroa MO, de Taddeo-Borg M, Kroon DE, Turner RS, Watson SJ, Margolis B. Molecular analysis of the XII-mLin-2/CASK complex in brain. J Neurosci Feb. 15, 1999;19(4):1307-1316.
De Strooper B, Annaert W, Cupers P, Saftig P, Craessaerts K, Mumm JS; Schroeter EH, Schrijvers V, Wolfe MS, Ray WJ, Goate A, Kopan R. A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain. Nature Apr. 8, 1999;398(6727):518-522.
Haass C, De Strooper B. The presenilins in Alzheimer's disease—proteolysis holds the key. Science Oct. 29, 1999;286(5441):916-919.
McLoughlin DM, Irving NG, Brownlees J, Brion JP, Leroy K, Miller CC. Mint2/XII-like colocalizes with the Alzheimer's disease amyloid precursor protein and is associated with neuritic plaques in Alzheimer's disease. Eur J Neurosci Jun. 1999;11(6):1988-1994.
Steiner H, Duff K, Capell A, Romig H, Grim MG, Lincoln S, Hardy J, Yu X, Picciano M, Fechteler K, Citron M, Kopan R, Pesold B, Keck S, Baader M, Tomita T, Iwatsubo T, Baumeister R, Haass C. A loss of function mutation of presenilin-2 interferes with amyloid beta-peptide production and notch signaling. J Biol Chem Oct. 1, 1999;274(40):28669-28673.
Struhl G, Greenwald I. Preseniln is required for activity and nuclear access of Notch in Drosophila. Nature Apr. 8, 1999;398(6727):522-525.
Whitfield CW, Benard C, Barnes T, Hekimi S, Kim SK. Basolateral localization of the Caenorhabditis elegans epidermal growth factor receptor in epithelial cells by the PDZ protein LIN-10. Mol Biol Cell Jun. 1999;10(6):2087-2100.
Ye Y, Lukinova N, Fortini ME.Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants. Nature Apr. 8, 1999;398(6727):525-529.
Borg JP, Straight SW, Kaech SM, de Taddeo-Borg M, Kroon DE, Karnak D, Turner RS, Kim SK, Margolis B. Identification of an evolutionarily conserved heterotrimeric protein complex involved in protein targeting. J Biol Chem Nov. 27, 1998;273(48):31633-31636.
Borg JP, Yan Y, De Taddeo-Borg M, Margolis B, Turner RS. The XIIalpha protein slows cellular amyloid precursor protein processing and reduces Abeta40 and Abeta42 secretion. J Biol Chem Jun. 12, 1998;273(24):14761-14766.
Butz S, Okamoto M, Sudhof TC. A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Cell Sep. 18, 1998;94(6):773-782.
Kaech SM, Whitfield CW, Kim SK. The LIN-2/LIN-7/LIN-10 complex mediates basolateral membrane localization of the C. elegans EGF receptor LET-23 in vulval epithelial cells. Cell Sep. 18, 1998;94(6):761-771.
Okamoto M, Südhof TC. Mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2. Eur J Cell Biol Nov. 1998;77(3):161-165.
Rongo C, Whitfield CW, Rodal A, Kim SK, Kaplan JM. LIN-10 is a shared component of the polarized protein localization pathways in neurons and epithelia. Cell Sep. 18, 1998;94(6):751-759.
Sastre M, Turner RS, Levy E. XII interaction with beta-amyloid precursor protein modulates its cellular stabilization and reduces amyloid beta-protein secretion. J Biol Chem Aug. 28, 1998:
Biederer Thomas
Ho Angela
Liu Xinran
Südhof Thomas C.
Board of Regents , The University of Texas System
Chernyshev Olga N.
LandOfFree
Methods for modulating transcriptional activation using mint... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for modulating transcriptional activation using mint..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for modulating transcriptional activation using mint... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3540614