Methods for screening of test compounds for inhibiting binding o

Details Methods for screening of test compounds for inhibiting binding o Methods for screening of test compounds for inhibiting binding o

1997-01-10

1998-08-25

Scheiner, Laurie

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 72, 435 724, C12Q 170, G01N 3353

Patent

active

057982066

ABSTRACT:
A method for the screening of a test compound for inhibiting the binding of a CD4-HIV 1 complex to HIV-1 second receptors, comprising: (a) preparing a magnetic ligand by mixing a magnetic, CD4-containing substrate with HIV-1, (b) mixing the magnetic ligand from step (a) with a test compound, (c) adding cells that express the HIV-1 second receptors to the mixture from step (b), (d) separating cells with bound magnetic ligands from cells without bound magnetic ligands by contact with a magnetic separator, and (e) quantifying the cells with bound magnetic ligands and quantifying the cells without bound magnetic ligands.

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