Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-08-16
2005-08-16
Leffers, Gerry (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S468000, C435S471000, C435S069100, C435S069700, C435S070100, C435S071100, C435S440000, C435S325000, C435S252100, C435S254200
Reexamination Certificate
active
06929917
ABSTRACT:
The invention concerns a new method of detecting a rare product of a directed genetic alteration of a cultured cell. The method is applicable to any method of making the alteration provided that a pair of closely linked alterations can be made. The method consists of sequentially using allele specific polymerase chain reaction (PCR) to preferentially amplify sequences containing one of the two linked alterations coupled with a second method that detects the second change in the PCR product. The second method can be restriction digestion, traditional sequencing or pyro-sequencing. Experiments indicate that alterations as rare as one correctly altered copy in 10,000 cells can be detected.
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Blaese R. Michael
DiCola Mike
Metz Richard
Akhavan Ramin
Fish & Neave IP Group
Leffers Gerry
PreGentis
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