Protein fragment complementation assays for the detection of...

Details Protein fragment complementation assays for the detection of... Protein fragment complementation assays for the detection of...

2005-08-16

2005-08-16

Brusca, John S. (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S320100, C530S350000, C536S023400

Reexamination Certificate

active

06929916

ABSTRACT:
The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule with the proviso that said protein is not ubiquitin.

REFERENCES:
patent: 6294330 (2001-09-01), Michnick et al.
patent: 6428951 (2002-08-01), Michnick et al.
patent: 2001/0047526 (2001-11-01), Brisson et al.
patent: 2003/0108869 (2003-06-01), Michnick et al.
Fields et al. The two-hybrid system: an assay for protein-protein interactions. Trends in Genetics vol. 10 pp. 286-292 (1994).
Johnsson et al. Split ubiquitin as a sensor of protein interactions in vivo. Proc. Natl. Acad. Sci. vol. 91 pp. 10340-10344.

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