Soluble thrombomodulin-based one-stage assay for vitamin-K depen

Details Soluble thrombomodulin-based one-stage assay for vitamin-K depen Soluble thrombomodulin-based one-stage assay for vitamin-K depen

1995-06-07

1996-06-11

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 4, 435 696, 436 63, 436 69, 514833, C12Q 156, C12Q 100, G01N 3348

Patent

active

055254780

ABSTRACT:
The present invention provides a novel one-stage assay which uses soluble thrombomodulin for directly determining the functional status in plasma of the vitamin K-dependent coagulation-inhibiting proteins, protein C and protein S. The method comprises combining a blood plasma test sample and a coagulation-activating substance in a calcium-free solution, then adding soluble thrombomodulin and calcium ions to initiate a coagulation reaction, measuring the time required for the test plasma sample to clot, comparing the measured clot time to that of standard control plasma samples, including protein C- and protein S-deficient plasma controls, and then using the compared clot time values for determining the functional status of protein C and protein S in the individual from whom the test plasma sample was taken. The functional status of the vitamin K-dependent coagulation-inhibiting proteins is used to determine the risk of thrombosis in the host individual.

REFERENCES:
patent: 4736018 (1988-04-01), Reutelingsperger
patent: 4748156 (1988-05-01), Aoki et al.
patent: 4849403 (1989-07-01), Stocker et al.
patent: 5001069 (1991-03-01), Bartl et al.
patent: 5051357 (1991-09-01), Hassovna
patent: 5120537 (1992-06-01), Esmon et al.
patent: 5147638 (1992-09-01), Esmon et al.
patent: 5279956 (1994-01-01), Griffin et al.
patent: 5330907 (1994-07-01), Philapitsch et al.
patent: 5426097 (1995-06-01), Stern
patent: 5439802 (1995-08-01), Rosen
patent: 5443960 (1995-08-01), Dahlback
patent: 5453373 (1995-09-01), Gerlitz et al.
Faioni et al, "Blood"; vol. 71(4), pp. 940-946, (Apr. 1988).

Affiliated with

Also associated with

Rating

Soluble thrombomodulin-based one-stage assay for vitamin-K depen does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Soluble thrombomodulin-based one-stage assay for vitamin-K depen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Soluble thrombomodulin-based one-stage assay for vitamin-K depen will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-350985