Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2005-08-09
2005-08-09
Achutamurthy, Ponnathapura (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S220000, C435S221000, C435S222000, C435S069100, C435S252300, C435S320100, C510S300000, C536S023200
Reexamination Certificate
active
06927055
ABSTRACT:
Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 ofBacillus amyloliquefacienssubtilisin.
REFERENCES:
patent: 4259357 (1981-03-01), Van Kraneneburg
patent: 4261868 (1981-04-01), Hora et al.
patent: 4404128 (1983-09-01), Anderson
patent: 4533359 (1985-08-01), Kondo et al.
patent: 4677272 (1987-06-01), Tajiri
patent: 4853871 (1989-08-01), Pantoliano et al.
patent: 4908773 (1990-03-01), Pantoliano et al.
patent: 5147642 (1992-09-01), Lotz et al.
patent: 5182204 (1993-01-01), Estell et al.
patent: 5204015 (1993-04-01), Caldwell et al.
patent: 5264366 (1993-11-01), Ferrari et al.
patent: RE34606 (1994-05-01), Estell et al.
patent: 5314692 (1994-05-01), Haarasilta et al.
patent: 5316935 (1994-05-01), Arnold et al.
patent: 5397705 (1995-03-01), Zukowski et al.
patent: 5534302 (1996-07-01), Ma et al.
patent: 5589373 (1996-12-01), Weiner et al.
patent: 5665587 (1997-09-01), Aaslyng et al.
patent: 5679630 (1997-10-01), Baeck et al.
patent: 5741664 (1998-04-01), Ballinger et al.
patent: 5741694 (1998-04-01), Hastrup et al.
patent: 5780285 (1998-07-01), Ballinger et al.
patent: 5837516 (1998-11-01), Ballinger et al.
patent: 5985639 (1999-11-01), Christianson et al.
patent: 6197567 (2001-03-01), Aaslyng et al.
patent: 6312963 (2001-11-01), Chou et al.
patent: 6482628 (2002-11-01), Poulose et al.
patent: 0134267 (1989-02-01), None
patent: 0 681 787 (1995-11-01), None
patent: WO 88 08165 (1988-10-01), None
patent: WO 91 00345 (1991-01-01), None
patent: WO 95 10615 (1995-04-01), None
patent: WO 96 28566 (1995-09-01), None
patent: WO 95 30010 (1995-11-01), None
patent: WO 95 30011 (1995-11-01), None
patent: WO 96 05739 (1996-02-01), None
*Poulos et al. (listed in spec as Robertus), “Polypeptide Halomethyl Ketones Bind to Serine Proteases as Analogs of the Tetrahedral Intermediate,”J. Biol. Chem., 251:1097-1103.
*Stauffer, et al., “The Effect on Subtilisin Activity of Oxidizing a Methionine Residue,”The Journal of Biological Chemistry, V. 244, N. 19, Issue of Oct. 10, pp. 5333-5338, 1969.
*Wells et al., “Subtilisin—an enzyme designed to be engineered,”Reviews TIBS, 13:291-297.
*Ballinger, M. D. et al: “Designing Substilisin BPN to Cleave Substartes Containing Dibasic Residues”Biochemistry, vol. 34, No. 41, pp13312-13319 (1995).
Baeck Andre C.
Caldwell Robert M.
Collier Katherine D.
Kellis, Jr. James T.
Nadherny Joanne
Achutamurthy Ponnathapura
Genencor International Inc.
Genencor International Inc.
Moore William W.
LandOfFree
Multiply-substituted protease variants does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multiply-substituted protease variants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multiply-substituted protease variants will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3496610