Ion-exchange resin/enzyme activity assay

Details Ion-exchange resin/enzyme activity assay Ion-exchange resin/enzyme activity assay

2005-08-09

2005-08-09

Gitomer, Ralph (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C435S004000

Reexamination Certificate

active

06927037

ABSTRACT:
The present invention relates to a rapid high-throughput ion-exchange resin assay for determining enzyme activity. This novel assay uses a radiometric technique which separates the radioactive substrate from the product (or the radioactive product from the substrate) by exploiting the differences in the net charges of the molecules using ion-exchange resin. This assay is useful, for example, for studies of enzyme kinetics, the identification of functional sites in the enzyme, and in the automated screening of compound libraries for pharmaceutical drug development.

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Sandmann G. Assays for Three Enzymes Involved in Mevalonic Acid Metabolism. Physiologia Plantarum 92(2)297-301, 1994.

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