Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Reexamination Certificate
2005-02-15
2005-02-15
Ludlow, Jan M. (Department: 1743)
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
C436S095000, C436S161000, C436S164000, C436S172000, C422S070000, C422S082050, C422S082080, C073S061560
Reexamination Certificate
active
06855555
ABSTRACT:
In conducting liquid chromatographic analysis of a saccharide mixture containing monosaccharides and oligosaccharides, elution is conducted by using two kinds or more of mobile phases to separate the saccharide mixture into individual constituent saccharides followed by conversion thereof into corresponding derivatives by reaction with reagents and detection of the derivatives as contained in a detector cell. The method of the invention is characterized in that the detector cell is cleaned by washing with a cleaning solvent after completion of the detection of each of the saccharide derivatives. The invention also provides an apparatus for efficiently conducting the above-mentioned inventive method comprising an analytical column, a reactor for converting the separated constituent saccharides into the derivatives, detector cell for containing the derivative, a detector for detecting the derivative contained in the detector cell, a solvent-feed means to introduce a cell-cleaning solvent into the cell and a flow channel-switching means.
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Kakita Hirotaka
Kamishima Hiroshi
Ludlow Jan M.
National Institute of Advanced Industrial Science and Technology
Wenderoth , Lind & Ponack, L.L.P.
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