Modified barley α-glucosidase

Details Modified barley α-glucosidase Modified barley α-glucosidase

2005-02-01

2005-02-01

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S004000, C435S006120, C435S069100, C435S183000, C435S200000, C435S210000, C435S440000, C435S455000, C435S468000, C536S023200

Reexamination Certificate

active

06849439

ABSTRACT:
Barley α-glucosidase is an important enzyme in the conversion of barley starch to fermentable sugars during the industrial production of ethanol, as in brewing and fuel ethanol production. The enzyme is, however, relatively thermolabile, a disadvantage for an enzyme useful in industrial processes which are preferably conducted at elevated temperatures. Site directed mutagenesis has been conducted to make mutant forms of barley α-glucosidase which have improved thermostability. The sites for this site-directed mutagenesis were selected by sequence comparisons with the sequences of other α-glucosidase proteins which are more thermostable. The recombinant mutant enzymes thus produced have been demonstrated to improve the thermostability of the enzyme.

REFERENCES:
patent: 5763252 (1998-06-01), Skadsen et al.
Frandsen et al. (Plant Physiol., 2000, vol. 123:275-282).*
Nakao et al. (Eur. J. Biochem., 1994, vol. 220(2):293-300).*
Scandurra et al. (Biochimie, 1998, vol. 80(11) :933-941).*
Li et al. (Protein Engg., 1997, vol. 10(10) :1199-1204).*
Igarashi et al. (Biosci. Biotechnol. Biochem., 1999, vol. 63(9): 1535-1540).

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