Method of producing an oil including docosahexaenoic acid

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S136000, C435S171000, C435S244000

Reexamination Certificate

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06977166

ABSTRACT:
C. cohniiis cultured in a suitable growth medium with acetic acid/acetate as the main carbon source. The acetate is provided, and replenished, by adding acetic acid to the growth medium in response to an increase in pH resulting from the utilisation of acetic acid/acetate byC. cohnii. TheC. cohniiproduces relatively high levels of docosahexaenoic acid (DHA). A stationary phase is not essential for satisfactory DHA production.

REFERENCES:
patent: 3444647 (1969-05-01), Takahashi
patent: 5130242 (1992-07-01), Barclay
patent: 5244921 (1993-09-01), Kyle et al.
patent: 5374657 (1994-12-01), Kyle
patent: 5397591 (1995-03-01), Kyle et al.
patent: 5407957 (1995-04-01), Kyle et al.
patent: 5429942 (1995-07-01), Kock et al.
patent: 5492938 (1996-02-01), Kyle et al.
patent: 5550156 (1996-08-01), Kyle
patent: 5567732 (1996-10-01), Kyle et al.
patent: 5658767 (1997-08-01), Kyle
patent: 5711983 (1998-01-01), Kyle et al.
patent: 0 515 460 (1992-12-01), None
patent: 7-87988 (1995-04-01), None
patent: WO 89/00606 (1989-01-01), None
patent: WO 96/40106 (1996-12-01), None
patent: WO 99/06585 (1999-02-01), None
patent: WO 00/54575 (2000-09-01), None
patent: WO 01/04338 (2001-01-01), None
patent: WO 01/49282 (2001-07-01), None
Vazhappilly et al., “Eicosapentaenoic Acid and Docosahexaenoic Acid Production Potential of Microalgae and their Heterotrophic Growth”, Journal of the American Oil Chemists, 75, No. 3, pp. 393-397 (1998).
Du Preez et al., “Production of Gamma-Linolenic Acid byMucor circinelloidesandMucor rouxiiwith Acetic Acid as Carbon Substrate”, Biotechnology Letters, 17, No. 9, pp. 933-938 (1995).
Vazhappilly et al., “Heterotrophic Production Potential of Omega-3 Polyunsaturated Fatty Acids by Microalgae and Algae-like Microorganisms”, Botanica Marina, 41, pp. 553-558 (1998).
Pringsheim, E.G., “Micro-Organisms From Decaying Seaweed”, Nature, 178, pp. 480-481 (1956).
Henderson et al., “Polyunsaturated Fatty Acid Metabolism in the Marine DinoflagellateCrypthecodinium cohnil”, Phytochemistry, 30, No. 6, pp. 1781-1787 (1991).
Sowers et al., “Growth of Acetotrophic, Methane-producing Bacteria in a pH Auxostat”, Current Microbiology, 11, pp. 227-230 (1984).
Tuttle et al., “An optimal growth medium for the dinoflagellateCrypthecodinium cohnil”, Phycologia, Blackwell Scientific Publ., 14:1, 1-8 (1975).
Martin et al., “A Method for the Regulation of Microbial Population Density during Continuous Culture at High Growth Rates”, Arch. Microbiol., 107, pp. 41-47 (1976).
Shahidi et al., “Omega-3 Fatty Acid Concentrates: Nutritional Aspects and Production Technologies”, Trends in Food Science & Technology, 9, pp. 230-240 (1998).
Oltmann et al., “Modification of the pH-Auxostat Culture Method for the Mass Cultivation of Bacteria”, Biotechnology and Bioengineering. vol. XX, pp. 921-925 (1978).
Provasoli et al., “Some Nutritional Characteristics ofGyrodinium cohnii, a Colorless Marine Dionflagellate”, Journal of Photozoology, vol. 4, Supplement, p. 7 (1957).
du Preez et al., “The Utilization of Short-Chain Monocarboxylic Acids as Carbon Sources for the Production of Gamma-Linolenic Acid byMucorStrains in Fed-batch Culture”, World Journal of Microbiology & Biotechnology, vol. 12, pp. 68-72, (1996).
A. G. Callely et al.; “The Metabolism of Propionate in the Colourless alga,Prototheca zopfii”, Biochem. Journal (1964), vol. 92, pp. 338-345.
Brands, S.J. (comp.); 1989-2004;Systema Naturae 2000. Amsterdam, The Netherlands.
Hastings et al.; “Qualitative Requirements and Utilization of Nutrients Algae”, CRC Press;Handbook Series in Nutrition and Food, Section D: Nutritional Requirements; (1977); vol. 1, pp. 87-163.
Lloyd, D., et al., “Propionate Assimilation in the FlagellatePolytomella caeca”, Biochem. J; (1968); vol. 109; pp. 897-907.
Smith et al.; “Metabolism of Propionate by Sensitive and Resistant Strains of Blue-Green Algae”;Biochemical Society Transactions; 537thMeeting, Canterbury, (1973); vol. 1, pp. 707-709.
Bahnweg; “Studies on the Physiology of Thraustochytriales, II. Carbon Nutrition of Thraustochytrium spp., Schizochtriym sp., Japanochytrium sp., Ulkenia spp., and Labyrinthuloides spp.”;Veroff. Inst. Meeresforsch. Bremerth; (1979); 17:pp. 269-273.
Bajpal et al.; “Production of Docosahenaenoic Acid by Thraustochytrium Aureum”;Applied Microbiology and Biotechnology; (1991); 35: pp. 706-710.
Beach et al.; “Biosynthesis of Oleic Acid and Docosahexaenoic Acid by a Heterotrophic Marine Dinoflagellate Crythecodinium Cohnil”;Biochimica et Biophysica Acta; (1974); 369: pp. 16-24.
Droop; “Algal Physiology and Biochemistry, Chapter 19: Heterotrophy of Carbon”;University of California Press; (1974); vol. 10; pp. 530-559.
Gold et al.; “Growth Requirements of Gyrodinium cohnil”;J. Protozool.; (1966); vol. 13, No. 2; pp. 255-257.
Hastings et al.; “Qualitative Requirements and Utilization of Nutrients: Algae”;Handbook Series in Nutrition and Food, Section D: Nutritional Requirements, CRC Press, Cleveland, Ohio; (1977); vol. 1; pp. 87-163.
Henderson et al.; “Lipid Composition and Biosynthesis in the Marine Dinoflagellate Crypthecodinium Cohnil”;Phytochemistry; (1988); vol. 27, No. 6; pp. 1679-1683.
Henderson et al.; “Polyunsaturated Fatty Acid Metabolism in the Marine Dinoflagellate Crypthecodinium Cohnil”; Biochemistry; (1991); vol. 30, No. 6; pp. 1781-1787.
Provasoli; “Marine Ecology, 5. Cultivation of Animals”;John Wiley & Sons; (1977); vol. 3; pp. 1295-1319.
Provasoli et al.; “Nutrition of the American Strain of Gyrodinium cohnil”;Archiv fur Mikrobiologie; 42; pp. 196-203.

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