Protease regulator screening assay

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S173300, C435S320100, C530S350000, C530S351000, C530S399000

Reexamination Certificate

active

06924122

ABSTRACT:
A polypeptide containing an anchor region, a protease recognition site, and a detectable signal region can be produced recombinantly and directly attached to a solid support. The polypeptide is useful for screening protease regulators, especially protease inhibitors.

REFERENCES:
patent: 4829009 (1989-05-01), Graves
patent: 5171662 (1992-12-01), Sharma
patent: 5227489 (1993-07-01), Law et al.
patent: 5292652 (1994-03-01), Dovey et al.
patent: 5347076 (1994-09-01), Pohlenz et al.
patent: 5386013 (1995-01-01), Lee et al.
patent: 5395752 (1995-03-01), Law et al.
patent: 5424205 (1995-06-01), Dovey et al.
patent: 5597691 (1997-01-01), Houghton et al.
patent: 0 196056 (1986-10-01), None
patent: 0 421109 (1991-04-01), None
patent: 0 428000 (1991-05-01), None
patent: 2276621 (1994-10-01), None
patent: WO 95/02065 (1995-01-01), None
patent: WO 95/22985 (1995-08-01), None
patent: WO 96/35717 (1996-11-01), None
patent: WO 96/36702 (1996-11-01), None
Rose and Craik, “Structure-Assisted Design of Nonpeptide Human Immunodeficiency Virus-1 Protease Inhibitors”, Am. J. Respir. Crit. Care Med., 1994, S176-S182, vol. 150(6, pt2).
Heldsinger and Antonucci, “An HIV-1 Protease Sveening Assay Using a Non-infectious Proviral Clone”, J. Virological Methods, 1994, 247-255, vol. 49.
Sudarsanam, et al., “An Approach to Computer-Aided Inhibitor Design: Application to Cathespin L”, J. Computer-Aided Molecular Design, 1992, 223-233, vol. 6.
Holskin, et al., “A Continuous Flourescence-Based Assay of Human Cytomegalovirus Protease Using a Peptide Substrate”, Analytical Biochemistry, 1995, 148-155, vol. 227.
Sato, et al., “A Simple and Rapid Method for Preliminary Evaluation of in Vivo Efficacy of Anti-HIV Compounds in Mice”, Antiviral Research, 1995, 151-163, vol. 27.
Hellen, “Assay Methods for Retroviral Proteases”, Methods in Enzymol., 1994, 46-58, vol. 241.
Sedlacek, et al., “A Rapid Screening Method for Biological Activity of Human Immunodeficiency Virus Proteinase Inhibitors by Using a Recombinant DNA-Derived Bacterial System”, Analytical Biochemistry, 1993, 306-309, vol. 215.
Yu, et al., “Assay of HIV-1 Protease Activity by Use of Crude Preparations of Enzyme and Biotinylated Substrate,” J. Virological Methods, 1995, 63-73, vol. 210(3).
Kakiuchi, et al., “Bacterial Expression and Analysis of Cleavage Activity of HCV Serine Proteinase Using Recombinant and synthetic Substrate”, Biochemical and Biophysical Research Communications, 1995, 1059-1065, vol. 210(3).
Mori, et al., “Enzymatic Characterization of Purified Ns3 Serine Proteinase of Hepatitis C Virus Expressed inEscherichia coli”, FEBS Letters, 1996, 37-42, vol. 378.
Steikuuhler, et al., Activity of Purified Hepatitis C Virus Protease NS3 on Peptide Substrates, Journal of Virology, Oct. 1996, 6694-6700.
Kim, et al., “Crystal Structure of the Hepatitis C Virus NS3 Protease Domain Complexed with a Synthetic NSA Cofactor Peptide”, Cell. Oct. 18, 1996, 348-355, vol. 87.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Protease regulator screening assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Protease regulator screening assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protease regulator screening assay will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3465633

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.