Compositions and methods for helper-free production of...

Details Compositions and methods for helper-free production of... Compositions and methods for helper-free production of...

2005-10-11

2005-10-11

Leffers, Gerry (Department: 1636)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S455000, C435S456000, C435S320100, C424S093200, C424S093210, C536S023100, C536S024100

Reexamination Certificate

active

06953690

ABSTRACT:
A method for producing recombinant adeno-associated virus in the absence of contaminating helper virus or wild-type virus involves culturing a mammalian host cell containing a transgene flanked by adeno-associated virus (AAV) inverse terminal repeats and under the control of regulatory sequences directing expression thereof, an AAV rep sequence and an AAV cap sequence under the control of regulatory sequences directing expression thereof, and the minimum adenovirus DNA required to express an E1a gene product, an E1b gene product and an E2a gene product, and isolating therefrom a recombinant AAV which expresses the transgene in the absence of contaminating helper virus or wildtype AAV. This method obviates a subsequent purification step to purify rAAV from contaminating virus. Also provided are various embodiments of the host cell.

REFERENCES:
patent: 5436146 (1995-07-01), Shenk
patent: 5622856 (1997-04-01), Natsoulis
patent: 5658776 (1997-08-01), Flotte et al.
patent: 5658785 (1997-08-01), Johnson
patent: 5681731 (1997-10-01), Lebkowski et al.
patent: 5756283 (1998-05-01), Wilson et al.
patent: 5837484 (1998-11-01), Trempe et al.
patent: 5856152 (1999-01-01), Wilson et al.
patent: 5871982 (1999-02-01), Wilson et al.
patent: 6251677 (2001-06-01), Wilson et al.
patent: 6258595 (2001-07-01), Gao et al.
patent: 6475769 (2002-11-01), Wilson et al.
patent: 6485966 (2002-11-01), Gao et al.
patent: 6541258 (2003-04-01), Allen et al.
patent: 2003/0040101 (2003-02-01), Wilson et al.
patent: 2003/0119191 (2003-06-01), Gao et al.
patent: 2004/0018627 (2004-01-01), Natsoulis et al.
patent: WO 95/06743 (1995-03-01), None
patent: WO 95/34670 (1995-12-01), None
patent: WO 96/13598 (1996-05-01), None
patent: WO 96/14061 (1996-05-01), None
patent: WO 96/39530 (1996-12-01), None
patent: WO 96/40240 (1996-12-01), None
patent: WO 97/12050 (1997-04-01), None
patent: WO 97/17458 (1997-05-01), None
patent: WO 97/26337 (1997-07-01), None
patent: WO 98/10086 (1998-02-01), None
patent: WO 98/09657 (1998-03-01), None
patent: WO 98/10088 (1998-03-01), None
patent: WO 99/14354 (1999-03-01), None
patent: WO 99/15685 (1999-04-01), None
patent: WO00/55342 (2000-09-01), None
Fields et al. Virology. vol. 2., 3rd edition. 1995. Philadelphia: Lippencott Willims and Wilkins; p. 2183.
Alkhatib et al. Journal of Virology. 1988; 62 (8): 2718-27, abstract only.
B. J. Carter, “The Growth Cycle of Adeno-Associated Virus”, inHandbook of Parvoviruses, ed. Tijsser, CRC Press, Quebec, Canada, pp. 155-168 (1990).
G-P. Gao, “High Titer Adeno-Associated Viral Vectors from a Rep/Cap Cell Line and Hybrid Shuttle Virus”,Human Gene Therapy, 9:2353-2362 (Nov. 1, 1998).
R. B. Kotin, “Prospects for the Use of Adeno-Associated Virus as a Vector for Human Gene Therapy”,Human Gene Therapy, 5:793-801 (Jul. 1994).
D. D. Coovert, “Gene Therapy for Muscle Diseases”,Current Opinion in Neurology, 7(5):463-470 (Oct. 1, 1994).
G-P. Gao et al., “Compositions and Methods for Helper-Free Production of Recombinant Adeno-Associated Viruses”, U. S. Appl. No. 09/404,555, filed Sep. 23, 1999.
Berendsen et al, “A Glimpse of the Holy Grail?”, Science, 282, pp. 543-642 (Oct. 1998).
Fisher et al, “Recombinant adeno-associated virus for muscle directed gene therapy”, Nature Medicine, 3(3): 306-312 (Mar. 1997).
Herzog et al, “Stable gene transfer and expression of human blood coagulation factor IX after intramuscular injection of recombinant adeno-associated virus”, Proc. Natl. Acad. Sci. USA, 94:5804-5809 (May 1997).
Kessler et al, “Gene delivery to skeletal muscle results in sustained expression and systemic delivery of a therapeutic protein”, Proc. Natl. Acad. Sci. USA, 93: 14082-14087 (Nov. 1996).
Samulski et al, “Helper-Free Stocks of Recombinant Adeno-associated Viruses: Normal Integration Does Not Require Viral Gene Expression”, J. Virol., 63(9): 3822-3828 (Sep. 1989).

Affiliated with

Also associated with

Rating

Compositions and methods for helper-free production of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Compositions and methods for helper-free production of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and methods for helper-free production of... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3455429