Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-09-06
2005-09-06
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S091510, C536S023100, C536S024300, C536S024330, C536S025300, C536S025410
Reexamination Certificate
active
06939671
ABSTRACT:
This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Extracellular RNA may circulate as non-bound RNA, protein-bound RNA, lipid-RNA complexes, lipoprotein (proteolipid)—RNA complexes, protein-RNA complexes including within or in association with ribonucleoprotein complexes, nucleosomes, or within apoptotic bodies. Any intracellular RNA found in plasma or serum can additionally be detected by this invention. Specifically, this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays for the identification, detection, inference, monitoring, or evaluation of any neoplasm, benign, premalignant, or malignant, in humans or other animals, which might be associated with that RNA. Further, this invention allows the qualitative or quantitative detection of tumor-derived or associated extracellular RNA circulating in the plasma or serum of humans or animals with or without any prior knowledge of the presence of cancer or premalignant tissue.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4699877 (1987-10-01), Cline et al.
patent: 4874858 (1989-10-01), Magistro
patent: 4999290 (1991-03-01), Lee
patent: 5087617 (1992-02-01), Smith
patent: 5098890 (1992-03-01), Gewirtz et al.
patent: 5124246 (1992-06-01), Urdea et al.
patent: 5155018 (1992-10-01), Gillespie et al.
patent: 5217889 (1993-06-01), Roninson et al.
patent: 5274087 (1993-12-01), Barnett et al.
patent: 5300635 (1994-04-01), Macfarlane
patent: 5409818 (1995-04-01), Davey et al.
patent: 5470724 (1995-11-01), Ahern
patent: 6329179 (2001-12-01), Kopreski
patent: 3717212 (1988-12-01), None
patent: WO 90/09456 (1990-08-01), None
Kelier et al., Fluorescence-based RT PCR analysis: determination of the ratio of soluble to membrane-bound forms of FcrRIIA transcripts in hematopoietic cell lines. PCR Methods and Applications, 3, 32-38, 1993.
Cheung et al., Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles. J. Clin. Microbiology, 32, 2593-2597, Oct. 1994.
Datta et al., Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction J. Clin. Oncology, 12, 475-482, 1994.
Kamm et al., Nucleic Acid concentrations in normal human plasma. Clinical Chem., 18, 519-522, 1972.
Moreno et al., Detection of hematogenous micrometastasis in patients with prostate cancer. Cancer Research, 52, 5110-5112, 1992.
Cheung et al., Rapid and sensitive method for detection of hepatitis C virus RNA by using silica particles. J. Clin. Microbiology, 32, 2593-2597, Oct. 1994.
Datta et al., Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction J. Clin. Oncology, 12, 475-482, 1994.
Wieczorek et al., Isolation and characterization of a RNA-proteolipid complex associated with the malignant state in human. Proc. Natl. Acad. Sci. USA, 82, 3455-3459, 1985.
Moreno et al., Detection of hematogenous micrometastasis in patients with prostate cancer. Cancer Research, 52, 5110-5112, 1992.
Abravaya et al., “Detection of point mutations with a modified ligase chain reaction (GAP-LCR),”Nucleic Acids Research23:675-682 (1995).
Alkema et al., “Characterization and Chromosamal Localization of the Human Prata-Oncogene BMI-1,”Human Mol Genet2:1597-1603 (1993).
Aoki et al., “Liposome-mediated in viva gene transfer on antisense K-ras construct inhibits pancreatic tumor dissemination in the murine peritoneal cavity,”Cancer Research55:3810-3816 (1995).
Barz et al., “Characterization of Cellular and Extracellular Plasma Membrane Vesicles from a Non-metastasing Lymphoma (Eb) and Its Metastasing Variant (Esb),”Biochin Biophys Acta814:77-84 (1985).
Bauer et al., “Identification of H-2Kb Binding and Immunogenic Peptides from Human Papillama Virus Tumour Antigens E6 and E7,”Scand J Immunol42:317-323 (1995).
Blackburn et al., “Electrochemiluminescence detection for development of immunoassays and clinical diagnostics,”Olin Chem37/9:1534-1539 (1991).
Bobo et al., “Diagnosis of chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay,”J din Micra28:1968-1973 (1990).
Bocchia et al., “Specific Binding of Leukemia Oncogene Fusion Peptides to HLA Class I Molecules,”Blood85:2680-2684 (1995).
Boom et al., “Rapid and Simple Method for Purification of Nucleic Acids,”J Clin Micro28:495-503 (1990).
Boom et al., “Rapid Purification of Hepatitis B Virus DNA from Seruc,”J Clin Micro29:180-181 (1991).
Brossart et al., “Detection of residual tumor cells in patients with malignant melanoma responding to immunotherapy,”J Immunotherapy15:38-41 (1994.
Buchman et al., “Selective RNA amplification: A novel method using d UMP-containing primers and uracil DNA glycosylase,”PCR Methods Applic3:28-31 (1993).
Carr et al., “Circulating Membrane Vesicles in Leukemic Blood,”Cancer Research45:5944-5951 (1985).
Cheung et al., “Rapid and Sensitive Method for Detection of Hepatitis C Virus RNA by Using Silica Particles,”J Clin Micro32:2593-2597 (1994).
Chrigwin et al., “Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease,”Biochemistry18:5294-5299 (1979).
Chomczynski and Mackey, “Modification of the TRI reagent (TM) procedure for isolation of RNA from polysaccaride- and proteaglycan-rich sources,”BioTechniques19:942-945 (1995).
Chomczynski and Mackey, “Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation,”Analytical Biochemistry225:163-164 (1995).
Chomczynski et al., “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,”Analytical Biochemistry162:156-159 (1987).
Chomczynski, “A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cells and tissue samples,”Biotech15:532-537 (1993).
Chu et al., “Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro,”Mol Cell Biol15:179-185 (1995).
Cohen, “Biochemical Therapy: Antisense Compounds,”In: Biologic Teraphy of Cancer(DeVita, Hellman, Rosenberg, eds) J.B. Lippincott, Ca., Philadelphia (1991) pp 763-775.
Colomer et al., “erB-2 antisense oligonucleotides inhibit the proliferation of breast carcinoma cells with erb-2 oncogene amplification,”Br J Cancer70:819-825 (1994).
Coutlee et al., “Immunodetection of DNA with biotinylated RNA probes: A study of reactivity of a monoclonal antibody to DNA-RNA hybrids,”Analytical Biochemistry181:96-105 (1989).
Datta et al., “Sensitive Detection of Occult Breast Cancer by the Reverse-transcriptase Polymerase Chain Reaction,”Journal of Clinical Oncology12:475-482 (1994).
Davidova and Shapot, “Liporibonucleoprotein Complex as an Integral Part of Animal Cell Plasma Membranes,”FEBS Lett6:349-351 (1970).
DiCesare et al., “A high-sensitivity electrochemiluminescence-based detection system for automated PCR product quantitation,”BioTechniques15:152-157 (1993).
Doi et al., “Detection of beta-human chorionic ganadotropin mRNA as a marker for cutaneoud malignant melanoma,”Int J Cancer65:454-45- (1996).
Dosaka et al., “A complex pattern of translational initiation and phosphorylation in L-Myc Proteins,”Oncogene6:371-378 (1991).
Edmands et al., “Rapid RT-PCR Amplification from Limited Cell Numbers,”PCR Methods Applic3:317-319 (1994).
Feng et al., “The RNA component of human telomerase,”Science269:1236-1241 (1995).
Fournie et al., “Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation,”Ana
Lu Frank
McDonnell & Boehnen Hulbert & Berghoff
Oncomedx, Inc.
Whisenant Ethan
LandOfFree
Method enabling use of extracellular RNA extracted from... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method enabling use of extracellular RNA extracted from..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method enabling use of extracellular RNA extracted from... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3446740