Methods and apparata for precisely dispensing microvolumes...

Fluid sprinkling – spraying – and diffusing – With means to vibrate or jiggle discharge

Reexamination Certificate

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C239S102200, C239S086000, C239S589100, C422S105000, C422S930000, C073S001740, C073S863320, C073S864010, C073S864220, C347S046000, C347S047000

Reexamination Certificate

active

06874699

ABSTRACT:
Devices and methods for depositing fluids on substrates in patterns of spots, lines, or other features use a nozzle, which is preferably configured similarly to a micropipette, having a piezoelectric crystal or other ultrasonic actuator coupled to one of its sides. The nozzle may be charged via capillary action by dipping it into a well containing the fluid to be deposited, and may then be positioned over a desired area of a substrate, at which point activation of the ultrasonic actuator at ultrasonic frequencies will eject the fluid onto the substrate. The needle may subsequently be dipped into a well of rinsing fluid for cleaning. Spots or lines on the order of 5 micrometers width may be generated, making the invention particularly suitable for use in biological applications such as microarray production and in microelectronics applications such as the printing of organic circuitry.

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