Expression cloning process for gene coding for a...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S029000

Reexamination Certificate

active

06846624

ABSTRACT:
The present invention provides a process for efficiently cloning DNA that codes for ligand-binding protein. This process is characterized in that a host containing DNA inserted into an expression vector is plate-cultured, said DNA is expressed, the protein that is produced is transferred to a film from said plate by placing said film in contact with said plate, said film is peeled from said plate, labeled ligand is added to said film to bind said ligand to said protein, the label bound to said protein is detected, and DNA is isolated from clones on the plate corresponding to locations on the film that demonstrated a positive reaction.

REFERENCES:
patent: 5665865 (1997-09-01), Lerner et al.
patent: 5679548 (1997-10-01), Barbas et al.
patent: 5759803 (1998-06-01), Kaelin et al.
patent: 5821070 (1998-10-01), Lee et al.
patent: 5891418 (1999-04-01), Sharma
patent: 5917018 (1999-06-01), Thogerson et al.
patent: 8909777 (1989-10-01), None
Reardan et al. Antibodies against metal chelates. Nature, vol. 316:265-267, 1985.*
Stemmer et al. Selection of an active single chain Fv antibody from a protein linker library prepared by enzymatic inverse PCR. BioTechniques vol. 14(2):256-265, 1993.*
Hoffman et al. Binding of antibodies and other proteins to nutrocellulose in acidic, basic, and chaotropic buffers. Anal. Biochem. vol. 198:112-118, Apr. 1991.*
Khoo et al. Purification and properties of rat cysteine-rich intestinal protein. Biochemical Journal. vol. 299:445-450, Sep. 1993.*
Sunderman et al. Xenopus lipovitellin 1 is a zn (2+) —and cd(2+) —binding protein. Molecular Reproduction And Develepment. vol. 42:180-187, Oct. 10, 1995.*
Pohl et al. Plaque-liftr testing of expression vector lambdagt11 with gold-labeled immunoglobulins. Anal. Biochem. vol. 175:414-421, Mar. 1988.*
Stone et al., “Targeted RNA Fingerprinting: The Cloning of Differentially-Expressed cDNA GB, Fragments Enriched for Members of the Zinc Finger Gene Family” Nucleic Acids Research, Oxford University Press, Surrey, vol. 22, No. 13, 1994, pp 2612-2618.
Skolnik et al., “Cloning of Pl3 Kinase-Associated p85 Utilizing a novel method for Expression/Cloning of Target Proteins for Receptor Tyrosine Kinases,”Cell(Apr. 5, 1991), vol. 65, pp. 83-90, XP-002100383.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Expression cloning process for gene coding for a... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression cloning process for gene coding for a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression cloning process for gene coding for a... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3435765

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.