Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-01-25
2005-01-25
McKelvey, Terry (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S029000
Reexamination Certificate
active
06846624
ABSTRACT:
The present invention provides a process for efficiently cloning DNA that codes for ligand-binding protein. This process is characterized in that a host containing DNA inserted into an expression vector is plate-cultured, said DNA is expressed, the protein that is produced is transferred to a film from said plate by placing said film in contact with said plate, said film is peeled from said plate, labeled ligand is added to said film to bind said ligand to said protein, the label bound to said protein is detected, and DNA is isolated from clones on the plate corresponding to locations on the film that demonstrated a positive reaction.
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