Polynucleotide separation method and apparatus therefor

Details Polynucleotide separation method and apparatus therefor Polynucleotide separation method and apparatus therefor

2005-05-17

2005-05-17

Forman, BJ (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S287200, C435S288200, C435S288400, C435S002000, C422S050000, C422S068100, C422S105000

Reexamination Certificate

active

06893823

ABSTRACT:
Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

REFERENCES:
patent: 4446237 (1984-05-01), Berninger
patent: 4893886 (1990-01-01), Ashkin et al.
patent: 5587128 (1996-12-01), Wilding et al.
patent: 5605662 (1997-02-01), Heller et al.
patent: 5607646 (1997-03-01), Okano et al.
patent: 5800992 (1998-09-01), Fodor et al.
patent: 6060288 (2000-05-01), Adams et al.
patent: 6280590 (2001-08-01), Cheng et al.
patent: WO 9642012 (1996-12-01), None
“Space-Resolved Control of Protein Subunit Dissociation/Association Using Laser Heating”, '94, Aug. 1994, M. Weshizu et al, pp. 111-114.
“Scanning Tunneling Microscopy of Mercapto-Hexyl-Oligonucleotides Attached to Gold”, Biophysical Journal, vol. 71, Aug. 1996, D. Rekesh et al, pp. 1079-1086.
“Electrostatic Manipulation of Bioparticles”, Kona, No. 14, M. Weshizu, pp. 61-71.
“Light-Directed, Spatially Addressable Parallel Chemical Synthesis”, Science, vol. 251, Feb. 15, 1991, Fodor et al, pp. 767-773.
“Remote Thermal Imaging With 0.7—um Spatial Resolution Using Temperature-Dependent Fluorescent Thin Films”, Applied Physical Letters, 42(1), Jan. 1, 1983, P. Kolodner et al, pp. 117-118.
“Covalent Attachment of Hybridizable Oligonucleotides to Glass Supports”, Analytical Biochemistry, vol. 247, 1997, B. Joos et al, pp. 96-101.
“Covalent Immobilization of DNA onto Polystyrene Microwells: The Molecules Are Only Bound at the 5 End”, Analytical Chemistry, 198, 1991, S. Rasmussen et al, pp. 138-142.
“DNA Analysis and Diagnostics on Oligonucleotide Microchips”, Proceedings National Academy of Science USA, 93, 1996, G. Yershov et al.

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