Method for isolating satellite sequences

Details Method for isolating satellite sequences Method for isolating satellite sequences

2005-07-19

2005-07-19

Leffers, Gerry (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S024300, C536S024330, C435S091400, C435S006120

Reexamination Certificate

active

06919443

ABSTRACT:
A highly homogeneous library can be obtained by cleaving a genomic DNA by a sequence-independent cleavage method, such as sonication. By selecting satellite sequences from such a library, efficiency of isolation is improved. Thus, an efficient method of isolating microsatellite sequences, which are useful as DNA markers, is provided.

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Burgtorf and Bunemann. A telomere-like satellite (GGGTCAT)n comprises 4% of genomic DNA ofDrosophila hydeiand is located mainly in centromeric heterochromatin of all large acrocentric autosomes. Gene (1993) vol. 137, pp 287-291.
Promega on-line catalog revised May 2000, DNA Polymerase I (Klenow) Fragment, Part# 9PIM220, copyright 1997-2000.
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