Purified Thermococcus barossii DNA polymerase

Details Purified Thermococcus barossii DNA polymerase Purified Thermococcus barossii DNA polymerase

1995-01-18

1997-02-11

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, 536 232, C12N 912, C12N 1554, C12P 1934

Patent

active

056020112

ABSTRACT:
A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90% activity after 8 hours of incubation at 95.degree. C. and retains between 60% and 80% proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.

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Uemori et al., Nucleic Acids Research, vol. 21, No. 2, pp. 259-265. 1993.
McPherson et al. "PCR, A Practical Approach", pp. 171-186. 1991.
Olga B. d'Hennezel, "Characterization of a Novel Thermostable DNA Polymerase from a Hydrothermal Submarine Vent Microorganism," North Carolina State University catalogued Apr. 6, 1994.

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