Method for specimen measurement

Details Method for specimen measurement Method for specimen measurement

1995-02-28

1997-02-11

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435961, 435968, 436518, 436524, 436528, 436533, 436536, 436542, 436172, 436800, 436804, 436805, 436823, 356337, 356441, C12Q 168

Patent

active

056019831

ABSTRACT:
In a method for measuring a specified component in a specimen by reacting the specimen with a first reagent formed by binding a substance active to the specified component, with carrier particles and a second reagent formed by labelling a substance active to the specified component with a first label, and measuring the substances in the complexes obtained in the reaction, there is disclosed a method featured by labelling the carrier particles with a second label different from the first label, and detecting the second label and then the first label utilizing the detection of the second label as a trigger.
This method enables a highly precise measurement without the influence of noise components in the detection of specified trace components in the specimen, utilizing an antigen-antibody reaction or a nucleic acid hybridization.

REFERENCES:
patent: 4385126 (1983-05-01), Chen et al.
patent: 4628037 (1986-12-01), Chagnon et al.
patent: 5123731 (1992-06-01), Yoshinaga et al.
patent: 5223398 (1993-06-01), Kortright et al.
Seradyn, Inc. "Microparticle Immunoassay Techniques" 2nd Ed. 1988.

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