Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-02
1997-04-22
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 43525231, 4353201, 435220, 4352523, 536 232, 536 237, 536 241, 935 14, 935 27, 935 29, 935 41, 935 48, 935 66, 935 74, C12N 954, C12N 1557, C12N 1574, C12N 1575
Patent
active
056228503
ABSTRACT:
The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.
REFERENCES:
patent: 4480037 (1984-10-01), Ichishima et al.
patent: 4559300 (1985-12-01), Kovacevic et al.
patent: 4652639 (1987-03-01), Stabinsky
patent: 4764470 (1988-08-01), Durham et al.
patent: 4771003 (1988-09-01), Stellwag et al.
patent: 5340735 (1994-08-01), Christianson et al.
Wells, J. A., et al., in The Genetics and Biotechnology of Bacilli, vol. 2, "Cloning and sequencing of a region controlling efficient expression of subtilisin from Bacillus amyloliquefaciens", pp. 173-180, 1983.
Wong, S.-L., et al., Proceedings of the National Academy of Sciences, U.S.A., vol. 81, "The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo", pp. 1184-1188 1984.
Jacobs, M., et al., Nucleic Acids Research, vol. 13, "Cloning, sequenceing and expression of subtilisin Carlsberg from Bacillus licheniformis", pp. 8913--8926.
Fortkamp, E., et al., DNA, vol. 5, "Cloning and expression in Escherichia coli of a synthetic DNA for hirudin, the blood coagulation inhibitor in the leech", pp. 511-517 1986.
Maciver et al., Applied and Environmental Microbiology, vol. 60, No. 11 pp. 3981-3988 (1994).
Aaslyng Dorrit A.
Dambmann Claus
Outtrup Helle
Sloma Alan P.
Agris, Esq. Cheryl H.
Moore William W.
Novo Nordisk A S
Novo Nordisk Biotech Inc.
Wax Robert A.
LandOfFree
Recombinant methods for the production of a bacillus alkaline pr does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant methods for the production of a bacillus alkaline pr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant methods for the production of a bacillus alkaline pr will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-340950