Catalase from bacillus and process for producing the same

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435183, 4352521, C12N 908

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active

056228490

ABSTRACT:
A microbial catalase having a catalase activity at 0.degree. C. of 95% or more of its catalase activity at 30.degree. C., when measured at pH 7, and a process for producing the same. The microbial catalase preferably has (1) an operative temperature of 0.degree. to 60.degree. C. and an optimum temperature of 0.degree. to 30.degree. C., when measured within the range of from 0.degree. to 60.degree. C., (2) an optimum pH of 7 to 10, (3) a resistance to 10 mM potassium fluoride, (4) a molecular weight is 65,000.+-.3,000, when measured by SDS polyacrylamide gel electrophoresis, and (5) an isoelectric point is about 4.8, when measured by isoelectric focusing.

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Ishida et al, Kumamoto J. Sci. Biol., 15(2):39-48 (1981).
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Lowen et al, Biochem. Cell Biol., 64:638-646 (1986).
Lowen et al, Biochem. Cell Biol., 65:939-947 (1987).
Lowen et al, Biochem. Cell Biol., 66:707-714 (1988).

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