Method of detecting DNA single strand breaks

Details Method of detecting DNA single strand breaks Method of detecting DNA single strand breaks

Filed on

2005-07-05

Date issued

2005-07-05

Examiner

Leffers, Gerry (Department: 1636)

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Measuring or testing process involving enzymes or...

Other Related Categories

C435S006120, C435S007200, C435S025000, C435S029000, C435S042000, C435S094000, C435S455000, C435S030000, C435S325000, C436S169000

Type

Reexamination Certificate

Status

active

Patent number

06913878

Description

ABSTRACT:
A method of detecting increased levels of DNA single strand breaks in a eukaryotic cell sample, comprising the steps of: (a) contacting a eukaryotic cell sample to a water-soluble tetrazolium salt under conditions in which said tetrazolium salt is converted to a formazan dye in said cell sample in the presence of NADH or NADPH; and then (b) detecting the presence of the formazan dye in said cell sample, with decreased levels of the formazan dye indicating increased levels of DNA single strand breaks in the eukaryotic cell sample.

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