Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-06-29
1997-04-22
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
540 1, 540450, 540451, 540452, 540455, 540465, 540467, 540476, 546 1, 546 2, 546 22, 546 23, 546 24, 546 26, 546 61, 549 1, 549 3, 549 5, 549 13, 549 23, 549200, 549204, 549206, 549218, 549220, 549263, 549273, 549283, 435 71, 435 72, 435810, 436501, 436546, 436536, 436 56, 436 63, 436 73, 436 81, 436 82, 436164, 436172, 436800, 436805, 530300, 530350, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168
Patent
active
056228210
ABSTRACT:
The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc.
The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.
REFERENCES:
patent: 4421654 (1983-12-01), Plueddemann
patent: 4637988 (1987-01-01), Hinshaw et al.
patent: 4794191 (1988-12-01), Hinshaw et al.
patent: 4801722 (1989-01-01), Hinshaw et al.
patent: 4837169 (1989-06-01), Toner
patent: 5021567 (1991-06-01), Johnson et al.
patent: 5252462 (1993-10-01), Drevin et al.
Ando et al., "Synthesis of a Highly Luminescent Terbium Chelate and its Application to Actin", Biochimica et Biophysica Acta 1102:186-194 (1992).
Baily et al., "Terbium Chelate for Use as a Label in Fluorescent Immunoassays", Analyst 109:1449-1450 (1984).
Canfi et al., "Fluorescent Terbium Chelates Derived From Diethylenetriaminepentaacetic Acid and Heterocyclic Compounds", Analyst 114:1405-1406 (1989).
Clark et al., "A Study of Sensitized Lanthanide Luminescence in an Engineered Calcium-Binding Protein", Analytical Biochemistry 210:1-6 (1993).
Diamandis, "Europium and Terbium Chelators as Candidate Substrates for Enzyme-labelled Time-resolved Fluorimetric Immunoassays", Analyst 117:1879-1884 (1992).
Georges and Ghazarian, "Study of Europium-sensitized Fluorescence of Tetracycline in a Micellar Solution of Triton X-100 by Fluorescence and Thermal Lens Spectrometry", Analytica Chimica Acta 276:401-409 (1993).
Mathis, "Rare Earth Cryptates and Homogeneous Fluoroimmunoassays with Human Sera", Clin. Chem. 39(9):1953-1959 (1993).
Saavedra and Picozza, "Time-resolved Fluorimetric Detection of Terbium-labelled Deoxyribonucleic Acid Separated by Gel Electrophoresis", Analyst 114:835-838 (1989).
Saha et al., "Time-Resolved Fluorescence of a New Europium Chelate Complex: Demonstration of Highly Sensitive Detection of Protein and DNA Samples", J. Am. Chem. Soc. 115(23):11032-11033 (1993).
Selvin et al., "Improved Fluorescence Energy Transfer Using Lanthanide Chelates", Biochemical Spectroscopy, a volume of Methods in Enzymology, Academic Press, Ed. Kenneth Sauer, vol. 246, pp. 300-334 (1995).
Seveus et al., "Time-resolved Fluorescence Imaging of Europium Chelate Label in Immunohistochemistry and In Situ Hybridization", Cytometry 13:329-338 (1992).
von Brenndorff et al., "Soft X-ray Stimulated High Resolution Luminescence Microscopy", Proceedings, 4th Intn'l Conf on X-ray Microscopy, Chernogolovoka, Moscow District, Russia (1993).
Hearst John
Selvin Paul R.
Marschel Ardin H.
Osman Richard Aron
The Regents of the University of California
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