Inducible promoters

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...

Reexamination Certificate

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C435S252300, C435S320100, C435S419000, C435S468000, C536S024100

Reexamination Certificate

active

06841720

ABSTRACT:
The present invention relates to inductible promoters for use in the control of heterologous genes in transformed plants. Suitable inducible promoters art those which are responsive to low levels of an environmentally acceptable and non-phytoxic inducing agent, and which also demonstrates a low level of developmentally or environmentally induced expression. A preferred prompter naturally drives the expression of a 21.3 kDa protein inAsparagus officinalisor an equivalent protein from the Lillaceae or Amaryllidaceae families. Under the control of a promoter of the invention, a gene will be expressed upon induction by SA or BTH, but preferably will not be developmentally expressed, systemically activated upon pathogen infection, or in response to ABA, ethylene, oxidative or osmotic stresses, or wounding.

REFERENCES:
patent: 0 332 104 (1989-09-01), None
patent: WO 9305164 (1993-03-01), None
patent: WO 9803536 (1998-01-01), None
Hennig et al. Pathogen, salicylic acid and developmental dependent expression of a beta-1,3-glucanase/GUS gene fusion in transgenic tobacco plants. Plant J. Sep. 1993;4(3):481-93.*
Menke et al. A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factorThe EMBO Journal, 1999, 18(16):4455-4463.*
Shah et al. Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related B-1,3-glucanase gene, PR-2d. The Plant Journal, 1996, vol. 10, No. 6, pp. 1089-1101.*
Görlach, J., et al., “Benzothiadiazole, A Novel Class of Inducers of Systemic Acquired Resistance, Activates Gene Expression and Disease Resistance in Wheat,”The Plant Cell 8:629-643, American Society of Plant Physiologists (1996).
International Search Report for Application No. PCT/GB99/01949, mailed Dec. 1999.

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