SMAD-interacting polypeptides and their use

Details SMAD-interacting polypeptides and their use SMAD-interacting polypeptides and their use

2005-04-26

2005-04-26

Carlson, Karen Cochrane (Department: 1653)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C530S300000, C530S350000

Reexamination Certificate

active

06884779

ABSTRACT:
The current invention concerns SMAD-interacting protein(s) obtainable by a two-hybrid screening assay whereby SMAD1 C-domain fused to GAL4 DNA-binding domain as “bait” and a cDNA library from mouse embryo as “prey” are used. Some characteristics of a specific SMAD-interacting protein (SIP1) of the family of zinc finger/homeodomain proteins including d-crystallin enhancer binding protein and/orDrosophilazfh-1 include an inability to interact with full-size XSMAD1 in yeast, SIP1CZFbinds to E2 box sites, SIP1CZFbinds to the Brachyury protein binding site and interferes with Brachyury-mediated transcription activation in cells and also interacts with the C-domain of SMAD 1, 2 and 5. The minimal length of the amino acid sequence necessary for binding with SMAD appears to be a 51 amino acid domain encompassing amino acids 166-216 of SEQ ID NO: 2 having the amino acid sequence as depicted in the one letter code: QHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKTEDISKLK (SEQ ID NO: 21).

REFERENCES:
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