Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2005-03-08
2005-03-08
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S478000, C435S320100
Reexamination Certificate
active
06864087
ABSTRACT:
A single-copy BAC vector (containing or lacking an insert) is converted in a host cell into a conditional high-copy BAC vector by introducing a conditional origin of replication into the single-copy BAC vector. The conditional ori is introduced by site-specific recombination between the SC BAC vector and a vector that contains the conditional ori. The host cell comprises a recombinase that recognizes a site-specific recombination site on both the BAC vector and the conditional ori vector. In the presence of the recombinase, the conditional ori-containing vector recombines into the BAC vector to produce a high-copy BAC vector that can be conditionally amplified by activating the conditional origin of replication on command.
REFERENCES:
patent: 5874259 (1999-02-01), Szybalski
patent: WO 0078977 (2000-12-01), None
Kim et al., Genome Research 8:404-412 (1998).
Hradecna Zdenka
Szybalski Waclaw
Wild Jadwiga
Ketter James
Quarles & Brady LLP
Vogel Nancy T.
Wisconsin Alumni Research Foundation
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